β-Adrenergic binding and secretory responses of the anterior pituitary

S. N. Perkins, W. S. Evans, M. O. Thorner, D. M. Gibbs, M. J. Cronin

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Our studies demonstrated that β-adrenergic agonists stimulate the release of GH from rat anterior pituitary (AP) cells in vitro. Concentration-response experiments with β-adrenergic agonists demonstrated that β2-adrenergic receptors mediated this phasic GH release, while having no apparent effect on PRL or LH release. The ACTH response to β-adrenergic agonists was equivocal. Half-maximal stimulation of GH release occured at 14 ± 2 (±SE) nM isoproterenol, 160 ± 30 nM epinephine, and over 1 μM l-norepinephrine (n = 4). Direct binding studies in membrane particulates of rat AP confirmed receptors of the β2-subtype. Iodocyanopindolol binding to β-adrenergic receptors or rat AP yielded a dissociation constant of 4.6 ± 0.1 pM and a maximal capacity of 1.9 ± 0.4 fmol/mg protein (n = 3). In contrast, porcine AP contained β1-adrenergic receptors. These results support the hypothesis that the endogenous β2-adrenergic agonist l-epinephrine may be a GH-releasing factor of physiological significance in the rat.

Original languageEnglish (US)
Pages (from-to)1818-1825
Number of pages8
JournalEndocrinology
Volume117
Issue number5
StatePublished - 1985
Externally publishedYes

Fingerprint

Adrenergic Agonists
Adrenergic Agents
Adrenergic Receptors
Iodocyanopindolol
Isoproterenol
Adrenocorticotropic Hormone
Epinephrine
Norepinephrine
Swine
Membranes
Proteins

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Perkins, S. N., Evans, W. S., Thorner, M. O., Gibbs, D. M., & Cronin, M. J. (1985). β-Adrenergic binding and secretory responses of the anterior pituitary. Endocrinology, 117(5), 1818-1825.

β-Adrenergic binding and secretory responses of the anterior pituitary. / Perkins, S. N.; Evans, W. S.; Thorner, M. O.; Gibbs, D. M.; Cronin, M. J.

In: Endocrinology, Vol. 117, No. 5, 1985, p. 1818-1825.

Research output: Contribution to journalArticle

Perkins, SN, Evans, WS, Thorner, MO, Gibbs, DM & Cronin, MJ 1985, 'β-Adrenergic binding and secretory responses of the anterior pituitary', Endocrinology, vol. 117, no. 5, pp. 1818-1825.
Perkins SN, Evans WS, Thorner MO, Gibbs DM, Cronin MJ. β-Adrenergic binding and secretory responses of the anterior pituitary. Endocrinology. 1985;117(5):1818-1825.
Perkins, S. N. ; Evans, W. S. ; Thorner, M. O. ; Gibbs, D. M. ; Cronin, M. J. / β-Adrenergic binding and secretory responses of the anterior pituitary. In: Endocrinology. 1985 ; Vol. 117, No. 5. pp. 1818-1825.
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