α-Hemoglobin-stabilizing protein is a sensitive and specific marker of erythroid precursors

Phil Raess, Michelle E. Paessler, Adam Bagg, Mitchell J. Weiss, John Kim Choi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

α-Hemoglobin-stabilizing protein (AHSP) is an abundant erythroid-specific chaperone protein that facilitates incorporation of nascent α-globin into hemoglobin A. We characterized AHSP expression by immunohistochemistry in a panel of 100 neoplastic and reactive bone marrow biopsies and splenic tissue with extramedullary hematopoiesis and compared it with established erythroid markers CD71 and CD235a. In all cases, AHSP expression was limited to physiological nucleated erythroid precursors (EPs) and blasts in erythroid leukemias. Although CD71 also stained EPs, it additionally stained nonerythroid malignant cells to varying extents in acute leukemia, diffuse large B-cell lymphoma, metastatic carcinomas, and small round blue cell tumors. In contrast, CD235a staining was erythroid-specific but stained non-nucleated red blood cells in all specimens, limiting its utility. We conclude that AHSP is superior to CD71 and CD235a for detecting normal and neoplastic nucleated EPs.

Original languageEnglish (US)
Pages (from-to)1538-1547
Number of pages10
JournalAmerican Journal of Surgical Pathology
Volume36
Issue number10
DOIs
StatePublished - Oct 1 2012
Externally publishedYes

Fingerprint

Leukemia
Hemoglobins
Extramedullary Hematopoiesis
Hemoglobin A
Globins
Lymphoma, Large B-Cell, Diffuse
Proteins
Erythrocytes
Bone Marrow
Immunohistochemistry
Staining and Labeling
Carcinoma
Biopsy
Neoplasms

Keywords

  • α-hemoglobin-stabilizing protein
  • CD71
  • erythroid precursor
  • immunohistochemistry

ASJC Scopus subject areas

  • Anatomy
  • Surgery
  • Pathology and Forensic Medicine

Cite this

α-Hemoglobin-stabilizing protein is a sensitive and specific marker of erythroid precursors. / Raess, Phil; Paessler, Michelle E.; Bagg, Adam; Weiss, Mitchell J.; Choi, John Kim.

In: American Journal of Surgical Pathology, Vol. 36, No. 10, 01.10.2012, p. 1538-1547.

Research output: Contribution to journalArticle

Raess, Phil ; Paessler, Michelle E. ; Bagg, Adam ; Weiss, Mitchell J. ; Choi, John Kim. / α-Hemoglobin-stabilizing protein is a sensitive and specific marker of erythroid precursors. In: American Journal of Surgical Pathology. 2012 ; Vol. 36, No. 10. pp. 1538-1547.
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