• Stouffer, Richard (PI)

    Project: Research project

    Project Details


    The proposed research is designed to test the hypothesis that the
    nonhuman primate (monkey) corpus luteum is a heterogeneous gland
    consisting of dynamic subpopulations of luteal cells which differ
    on the basis of structure, function and regulation by hormonal
    substances. The long-term objectives include elucidation of the
    origin(s), paracrine interactions and regulatory mechanisms for
    luteal cell subtypes which combine to produce the normal activity
    and lifespan of the primate corpus luteum. The initial aims are:
    (1) to distinguish and separate luteal cells into highly-enriched
    subpopulations based on differences in physical properties (cell
    size and organelles) and in binding of fluorescent-tagged hormones,
    (2) to characterize the ability of purified cell populations to
    produce steroid hormones and prostaglandins and to respond to
    luteotropic and luteolytic agents, and (3) to begin in vitro
    studies on possible cell-to-cell interactions influencing the
    steroidogenic activity of luteal cells. Collagenase-dispersed cells will be prepared from the corpus luteum
    of rhesus monkeys at midluteal phase of the menstrual cycle.
    Subpopulations of cells will be distinguished and separated by
    multiparameter flow cytometry, based on differences in forward and
    90 degrees light scatter (a function of cell size/shape and density
    of organelles) and the binding of fluorescent-tagged hCG and
    prostaglandins. Sorted groups of cells will be incubated in vitro
    as acute suspensions to examine (a) their capacity to produce
    progesterone, estrogens and prostaglandins (PGs) E2, F2 alpha, D2
    and I2, and (b) their sensitivity to gonadotropic hormones (LH and
    CG), various PGs and estrogens. Finally, different groups of cells
    will be cultured individually or together to elucidate cell-to-cell
    interactions which promote or inhibit steroidogenesis. Distinguishing the activities and regulatory mechanisms for luteal
    cell subtypes within the monkey corpus luteum could revise
    dramatically theories on the control of luteal function in
    primates. The project would provide the framework for further
    studies elucidating the origins, functions and changes in specific
    cell types during the lifespan of the corpus luteum in the normal
    menstrual cycle, throughout pregnancy and in situations associated
    with luteal dysfunction.
    Effective start/end date9/30/8811/30/02


    • National Institutes of Health: $276,299.00
    • National Institutes of Health: $284,589.00


    • Medicine(all)


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