STAGE-SPECIFIC GENES IN LEISHMANIA ENRIETTII

Project: Research project

Description

Leishmania parasites are the causative agents of leishmaniasis, a
disease that affects large numbers of people over vast regions of
the globe. There are two principal stages in the life cycle of
these parasitic protozoa: promastigotes are slender, flagellated,
extracellular organisms that live in the gut of the sandfly vector,
while amastigotes are oval, non-flagellated, sessile, intracellular
parasites that are specifically adapted for growth and survival
within the mammalian host macrophages. Each of these two
stages of the life cycle expresses a subset of stage-specific genes
that confer a distinct phenotype upon that stage. The purpose of this proposal is to study the genes that encode
three previously identified stage-specific proteins. The RNAs
encoding two of these proteins are very abundant in the
promstigote stage but are absent in amastigotes; the RNA
encoding the third protein is abundant in amastigotes but absent
in promastigotes. We will clone the genes encoding these three
stage-specific proteins and use these cloned genes to: i)
understand the molecular mechanisms responsible for repressing
their expression in one life-cycle stage and inducing their
expression in the other stage, and ii) probe the function of each
stage-specific protein in the parasite life cycle. These studies on stage-specific gene expression will contribute to
two long-term objectives: i) to understand basic features of gene
expression and regulation in these parasites, phenomena which are
poorly understood at present; ii) to understand how these parasites
adapt to and survive within two different and potentially hostile
environments, the insect gut and the host macrophage.
StatusFinished
Effective start/end date9/1/885/31/12

Funding

  • National Institutes of Health: $339,750.00
  • National Institutes of Health
  • National Institutes of Health: $137,689.00
  • National Institutes of Health: $275,514.00
  • National Institutes of Health
  • National Institutes of Health: $305,775.00
  • National Institutes of Health: $366,733.00
  • National Institutes of Health: $193,046.00
  • National Institutes of Health: $339,750.00
  • National Institutes of Health
  • National Institutes of Health: $366,733.00
  • National Institutes of Health: $286,534.00
  • National Institutes of Health: $383,541.00
  • National Institutes of Health: $166,560.00
  • National Institutes of Health
  • National Institutes of Health: $363,066.00
  • National Institutes of Health: $136,797.00
  • National Institutes of Health
  • National Institutes of Health: $339,750.00
  • National Institutes of Health: $373,734.00
  • National Institutes of Health: $339,750.00
  • National Institutes of Health
  • National Institutes of Health

Fingerprint

Leishmania enriettii
Leishmania
Parasites
parasites
Facilitative Glucose Transport Proteins
transporters
genes
amastigotes
Life Cycle Stages
nucleosides
insects
Membrane Transport Proteins
amino acid transporters
proteins
life cycle (organisms)
promastigotes
Glucose
Protein Isoforms
developmental stages
Nucleoside Transport Proteins

ASJC

  • Medicine(all)
  • Immunology and Microbiology(all)