SALMONELLA GENES REQUIRED FOR SURVIVAL

  • Heffron, Fred (PI)

    Project: Research project

    Project Details

    Description

    Intracellular pathogens, such as Salmonella typhimurium, can survive within
    a macrophage and have evolved a number of mechanisms to resist the
    bacteriocidal mechanisms within these professional phagocytes. We have
    developed a simple in vitro assay and used it to identify Salmonella
    mutants that cannot survive within the macrophage. Roughly 1% of the Tn10
    insertion mutants we have screened with this assay are not able to survive
    in the macrophage and all of these are avirulent as expected for a mutation
    in a known virulence mechanism. Secondary phenotypes have been assigned to
    46 mutants identified in this screen. Many of the mutations are located in
    pathways that would be anticipated to be essential for survival in the
    macrophage (eg. sensitivity to oxidants) whereas others either have no
    secondary phenotypes or were not expected (sensitivity to complement
    components). Understanding of the defects in these mutants will contribute
    to the understanding of other clinically important and more complex
    intracellular pathogens.
    StatusFinished
    Effective start/end date4/1/8711/30/13

    Funding

    • National Institutes of Health
    • National Institutes of Health: $268,655.00
    • National Institutes of Health: $464,054.00
    • National Institutes of Health: $352,944.00
    • National Institutes of Health: $344,135.00
    • National Institutes of Health: $334,110.00
    • National Institutes of Health
    • National Institutes of Health: $423,256.00
    • National Institutes of Health: $334,654.00
    • National Institutes of Health: $344,650.00
    • National Institutes of Health
    • National Institutes of Health: $289,404.00
    • National Institutes of Health: $421,326.00
    • National Institutes of Health
    • National Institutes of Health: $423,704.00
    • National Institutes of Health
    • National Institutes of Health: $122,488.00
    • National Institutes of Health
    • National Institutes of Health: $353,415.00
    • National Institutes of Health: $226,604.00
    • National Institutes of Health
    • National Institutes of Health: $327,729.00
    • National Institutes of Health
    • National Institutes of Health
    • National Institutes of Health: $396,047.00
    • National Institutes of Health: $188,750.00
    • National Institutes of Health
    • National Institutes of Health: $315,942.00

    ASJC

    • Medicine(all)
    • Immunology and Microbiology(all)

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