RENAL EPITHELIAL CELL RESPONSE TO UREA

Project: Research project

Description

The renal medulla is the only mammalian tissue exposed to elevated
concentrations of urea, a potent denaturant of proteins and nucleic acids
that adversely affects cell function. Renal epithelial cells in culture,
however, appear to be uniquely capable of responding to hyperosmotic urea
with mitogenesis and increased expression of two immediate-early gene
(IEG) transcription factors, Egr-1 and c-fos. As such, the overall
objective of this project is two-fold: to define the molecular mechanism
of urea-induced IEG expression; and to determine whether urea exerts its
renal epithelial cell-specific mitogenic effect through IEG-mediated
induction and autocrine action of a peptide growth factor or receptor.
StatusFinished
Effective start/end date7/1/936/30/99

Funding

  • National Institutes of Health: $93,312.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $32,498.00

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Immediate-Early Genes
Urea
Epithelial Cells
Kidney
Genetic Transcription
Growth Factor Receptors
Transcription Factors
Cell Culture Techniques
Gene Expression
Peptides
Proteins
Nucleic Acids

ASJC

  • Medicine(all)