REGULATION OF INSULIN-LIKE GROWTH FACTOR I EXPRESSION

  • Rotwein, Peter (PI)

    Project: Research project

    Project Details

    Description

    The goals of the investigation are to study the control of insulin-like
    growth factor I (IGF I) biosynthesis in model cell culture systems and in
    the whole animal. Based on our preliminary observations the human IGF I
    gene gives rise by alternative RNA processing to two messenger RNA
    transcripts encoding different peptide precursors. These initial studies
    suggest that regulatory mechanisms controlling IGF I biogenesis include
    both tissue-specific RNA splicing and tissue-specific protein processing.
    The putative regulatory steps may be responsible for unique IGF I peptide
    species subserving different roles as either hormones or
    autocrine/paracrine growth stimulators. In order to study the regulation
    of IGF I biosynthesis, I propose the following three specific objectives:
    (1) To define the protein precursors, processing intermediates, and steps
    leading to the secretion of mature IGF I by using the techniques of gene
    transfer to introduce IGF I complementary DNAs (cDNAs) into hepatocyte and
    fibroblast cell lines, and an inducible gene expression system to amplify
    IGF I biosynthesis. (2) To determine by molecular cloning the structure
    and sequence of rat IGF I messenger RNAs and gene. (3) To study the
    regulation of IGF I gene expression during growth and development in the
    rat using the homologous cDNAs and gene as probes, in particular to analyze
    the role of growth hormone in enhancing IGF I biosynthesis.
    StatusFinished
    Effective start/end date9/1/863/31/03

    Funding

    • National Institutes of Health
    • National Institutes of Health: $1,661.00
    • National Institutes of Health: $172,407.00
    • National Institutes of Health: $177,109.00
    • National Institutes of Health
    • National Institutes of Health
    • National Institutes of Health: $271,577.00
    • National Institutes of Health
    • National Institutes of Health
    • National Institutes of Health
    • National Institutes of Health: $276,768.00
    • National Institutes of Health
    • National Institutes of Health
    • National Institutes of Health
    • National Institutes of Health
    • National Institutes of Health: $200,505.00
    • National Institutes of Health: $266,536.00

    ASJC

    • Medicine(all)

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