REGULATION OF CAPSULE SYNTHESIS IN A PATHOGENIC E. COLI ISOLATE

  • Ullman, Buddy, (PI)
  • Russo, T. (PI)

Project: Research project

Description

Escherichia coli is the most common cause of urinary tract infection.
Its pathogenicity ranges from uncomplicated urethritis or cystitis to
pyelonephritis with sepsis and endotoxemic shock. Capsular
polysaccharides are believed to be important virulence determinants in a
variety of microorganisms. In E. coli capsule has been attributed to
play a role in the avoidance of killing by both complement and
phagocytic cells. The majority of studies on capsular regulation,
however, has been done on the group 1 capsule. Therefore we are
undertaking studies in a pathogenic K54 isolate to gain insight on the
regulation of group 2 capsule production. A more complete understanding
of regulation of capsular synthesis may lead to improved or novel
modalities for therapeutic intervention. Our approach in studying this
question will be to use a combination of gene disruption and
complementation experiments to evaluate the role, if any, of group 1
regulatory elements in the regulation of K54 (group 2) capsule
synthesis. Once this is established we will be able to identify any
novel regulatory proteins present in this pathogenic isolate.
StatusFinished
Effective start/end date9/1/8410/31/85

Funding

  • National Institutes of Health

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Leishmania
Capsules
Virulence
Escherichia coli
Urethritis
Cystitis
Urinary Tract Infections
Shock
Sepsis
Genes
purine
Proteins
Therapeutics

ASJC

  • Medicine(all)
  • Immunology and Microbiology(all)