PITUITARY GLYCOPROTEIN HORMONE GENE EXPRESSION

Project: Research project

Description

The overall goal of the proposed research is to understand the molecular
events which permit the regulated, coordinate expression of the pituitary
glycoprotein hormone genes, lutropin (LH), follitropin (FSH) and
thyrotropin (TSH). Besides being important physiologically for
reproduction and metabolic regulation, these proteins provide a good model
system for analysis of gene expression of subunit proteins. All three
proteins contain a common alpha subunit and a different beta subunit.
Initially cDNAs will be isolated for FSH-beta; cDNAs are already available
for the other subunits. The cloned cDNAs will be used to study the
regulation of subunit mRNA levels, gene transcription and gene structure.
Particular attention will be focused on the relationship of alpha subunit
gene structure and expression to the structure and expression of the beta
subunits. In addition, the interaction of steroid and thyroid hormone
receptors with the subunit gene sequences will be examined to gain further
insight into DNA sequences possibly involved in regulating gene
expression. The function of putative regulatory DNA sequences will then be
tested by construction of fusion genes which will be transfected into
suitable cells. Finally, an effort will be made to identify protein
factors which interact with the glycoprotein hormone genes to facilitate
their appropriate expression. These studies should provide increased
understanding of the molecular machinery responsible for the regulated
expression of the genes for subunit proteins.
StatusFinished
Effective start/end date2/1/866/30/01

Funding

  • National Institutes of Health: $237,557.00
  • National Institutes of Health
  • National Institutes of Health: $188,527.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $170,723.00

Fingerprint

Pituitary Hormones
Glycoproteins
Gene Expression
Hormones
Genes
Gonadotropin-Releasing Hormone
Follicle Stimulating Hormone
Response Elements
Luteinizing Hormone
Proto-Oncogene Proteins c-raf
Complementary DNA
Trans-Activators
Proteins
Sequence Deletion
Alpha Subunit Glycoprotein Hormones
Beta Subunit Follicle Stimulating Hormone
DNA
Transcription Factors
Carrier Proteins
Gene Fusion

ASJC

  • Medicine(all)