PEPTIDE SECONDARY METABOLITE BIOSYNTHESIS IN BACILLUS

Project: Research project

Description

Unicellular organisms as well as the cells of higher organisms utilize low
molecular weight polypeptides as extracellular signals that regulate cell
differentiation and specialization. Spore- forming bacteria of the genus
Bacillus are known to produce a number of peptides which possess
antimicrobial activity. More recently, evidence has emerged which
implicates these peptides as regulators of sporulation and competence
development. Surfactin is a cyclic lipopeptide and powerful biosurfactant
produced by B. subtilis. Genes required for surfactin production have been
identified and isolated. These include the srfA operon, which encodes at
least one of the enzymes involved in the synthesis of surfactin. Genetic
evidence has shown that srfA is also required for competence development
and efficient sporulation, which suggests that peptides synthesized by the
srfA enzymes may function in developmental processes. Transcription of
srfA is activated in stationary phase cells and is repressed by readily
metabolized carbon and nitrogen sources. Production of surfactin also
requires the comA gene, a putative transcriptional regulator of competence
gene and srfA transcription. The sfp gene is also required for surfactin
production, but its primary function of is unknown. The goals of the
experiments described herein is to identify and characterize the products
of the srfA and sfp genes, and to elucidate the mechanisms of srfA
transcriptional regulation. Genes that require srfA for their expression
will also be identified in order to begin to examine the role of srfA in
Bacillus development.
StatusFinished
Effective start/end date2/1/927/31/15

Funding

  • National Institutes of Health: $352,930.00
  • National Institutes of Health: $121,071.00
  • National Institutes of Health: $218,966.00
  • National Institutes of Health: $55,386.00
  • National Institutes of Health: $353,721.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $385,103.00
  • National Institutes of Health: $286,851.00
  • National Institutes of Health: $379,643.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $436,746.00
  • National Institutes of Health: $353,189.00
  • National Institutes of Health
  • National Institutes of Health: $286,851.00
  • National Institutes of Health: $286,851.00
  • National Institutes of Health: $281,963.00
  • National Institutes of Health
  • National Institutes of Health: $352,890.00
  • National Institutes of Health: $396,453.00
  • National Institutes of Health

Fingerprint

Operon
Mental Competency
DNA-Directed RNA Polymerases
Genes
Bacteria
Peptides
Bacillus subtilis
Proteins
Proteolysis
Oxidants
Oxidation-Reduction
Oxidative Stress
Coma
Bacillus
Poisons
DNA
Enzymes
Starvation
ATP-Dependent Proteases
Gene Expression

ASJC

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)