Molecular displacement in brain extracellular space

Project: Research project

Project Details


DESCRIPTION (Provided by Applicant): Clinical MRI studies correlate decreases
in molecular diffusion to abnormal regions within brain tissue. As a result,
diffusion weighed MRI is exquisitely sensitive to brain pathologies such as
stroke. The aim of this proposal is to elucidate determinants of molecular
diffusion in the extracellular space of brain and to clarify the factors
important for comparing diffusion measurements derived from different
experimental techniques. The two primary methods that generate diffusion
measurements in brain tissue are iontophoresis and magnetic resonance (MR)
spectroscopy. Although both methods have been extensively used by multiple
research groups to generate quantitative measurements in normal brain and in
many pathological brain states, comparisons between the two bodies of research
are complicated by the unknown effects of differences in the experimental
methodologies. This proposal outlines a strategy for calibrating the two
methods. The fundamental experimental involves measuring diffusion of the same
molecule with both techniques. After initial comparisons are made, the MR
diffusion measurements will be altered so that they are sensitive to motions
over a mean distance that approaches the distance between the electrodes used
for the iontophoresis measurements. Finally, the change in the observed
diffusion rates will be assessed under ischemic conditions to assess the degree
to which both diffusion measurements respond to the same anatomical changes at
the onset of ischemia.
Effective start/end date1/1/02 → …


  • National Institutes of Health: $46,420.00
  • National Institutes of Health: $38,320.00
  • National Institutes of Health: $48,928.00


  • Medicine(all)
  • Neuroscience(all)


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