IDENTIFICATION OF RECENT THYMIC EMIGRANTS IN THE HUMAN

Project: Research project

Description

DESCRIPTION: Dr. Picker proposes to develop two new assays for quantitating recent thymic emigrants in humans, the detection of T cell receptor rearrangement excision circles and the use of multiparameter flow cytometry to detect unique cell surface phenotypes. The first assay measures very common TC rearrangement-generated episomal circles from the TCR-delta rec locus to pseudo-J-alpha region, and the second assay measures CD8+ T cells that are CD103+, CD27 bright, CD95 dim, CD45RA+/RO-, Cdlla dim, and CD62L bright. When these assays are standardized, they will be applied to patients on HAART to monitor the level of thymic output of naiDve T cells.
StatusFinished
Effective start/end date9/30/989/29/01

Funding

  • National Institutes of Health
  • National Institutes of Health: $234,000.00
  • National Institutes of Health

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T-Lymphocytes
Highly Active Antiretroviral Therapy
T-Cell Antigen Receptor
Flow Cytometry
Phenotype

ASJC

  • Medicine(all)
  • Immunology and Microbiology(all)