GROWTH FACTORS IN PROLIFERATIVE VITREORETINOPATHY

Project: Research project

Description

Proliferative Vitreoretinopathy (PVR) is characterized by
proliferation of retinal pigment epithelial (RPE) cells,
fibroblasts and glial cells in the periretinal space following
trauma. The proliferation of these cells results in the formation
of a membrane which causes puckering and/or retraction of the
retina. PVR is the major complication arising from retinal re-
attachment surgery. Polypeptide growth factors control the rate of proliferation in
normal human cells in a paracrine manner. In proliferative
diseases, endogenous production of and response to growth factors
may lead to increased rates of cellular proliferation in an
autocrine fashion. In appropriate production of polypeptide growth
factors by RPE cells which migrate into the periretinal space in
PVR could contribute to the pathology of this disease.
Alternatively, the cells which proliferate in PVR could respond to
growth factors normally present in the retina. Therefore, the
specific aims of this project include: 1) The development of a
defined serum-free medium for the growth of RPE cells in vitro; 2)
Expanded studies on the production of growth factors by cultured
RPE cells; 3) Determining whether growth factors produced by RPE
cells can stimulate the proliferation of RPE cells and fibroblasts
in vitro; 4) Histological examination of normal and pathological
specimens to determine the distribution and synthesis of growth
factors in the posterior chamber of the eye.
StatusFinished
Effective start/end date5/1/894/30/93

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

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Proliferative Vitreoretinopathy
Retinal Pigments
Intercellular Signaling Peptides and Proteins
Epithelial Cells
Cell Proliferation
Peptides
Serum-Free Culture Media
Growth
Neuroglia
Retina
Fibroblasts
Pathology
Membranes

ASJC

  • Medicine(all)