GONADOTROPIN RECEPTORS OF THE PRIMATE CORPUS LUTEUM

    Project: Research project

    Project Details

    Description

    The proposed research is designed to test further the hypothesis that
    modulation of the gonadotropin receptor-adenylyl cyclase system is an
    important mechanism regulating corpus luteum function in primates during
    the menstrual cycle and early pregnancy. Based on progress in this
    laboratory, specific aims are presented: (a) to determine whether
    bona-fide receptors for luteinizing hormone (LH) and chorionic gonadotropin
    (CG) are masked in luteal membranes and to identify conditions which
    mask/unmask receptors, (b) to correlate the number of available, occupied,
    and masked LH-CG receptors with the functional state of the corpus luteum
    during the menstrual cycle, (c) to investigate in vitro the direct effects
    of potential luteolytic agents on the gonadotropin receptor-adenylyl
    cyclase system, (d) to compare the effect of in vivo exposure to hCG
    (designed to mimic early pregnancy levels) on gonadotropin
    receptor-adenylyl cyclase activity in young vs. mature corpora lutea, and
    (e) to develop an in vivo model examining the ability of intraovarian
    estrogen and prostaglandin to modulate gonadotropin receptor-adenylyl
    cyclase activity and the function of the primate corpus luteum. The adult,
    female rhesus monkey continues as the animal choice for our studies on the
    primate corpus luteum, with comparative studies employing luteal tissue
    from the pseudopregnant rat and the pig. Available and masked receptors
    will be characterized via specific 125I-labeled hLH/hCG binding to macaque
    luteal preparations before and after exposure to unmasking agents (e.g.,
    ethanol). Adenylyl cyclase activity will be assessed from the conversion
    of [Alpha32P] ATP to [Alpha32P] cyclic AMP by luteal preparations. These
    studies will provide new information on the cellular mechanims regulating
    the corpus luteum during the menstrual cycle and early pregnancy, with
    application to the control of human fertility and infertility. In
    particular, the investigation will determine whether heterologous and
    homologous regulation of the gonadotropin receptor-adenylyl cyclase system,
    by potent luteolytic agents and gonadotropins, respectively, plays an
    important role in the regulation of the function/lifespan of the primate
    corpus luteum.
    StatusFinished
    Effective start/end date7/1/852/28/16

    Funding

    • National Institutes of Health: $318,941.00
    • National Institutes of Health: $534,419.00
    • National Institutes of Health: $373,937.00
    • National Institutes of Health
    • National Institutes of Health
    • National Institutes of Health: $511,541.00
    • National Institutes of Health: $528,441.00
    • National Institutes of Health
    • National Institutes of Health
    • National Institutes of Health
    • National Institutes of Health
    • National Institutes of Health: $357,750.00
    • National Institutes of Health: $357,750.00
    • National Institutes of Health
    • National Institutes of Health
    • National Institutes of Health: $546,789.00
    • National Institutes of Health: $391,210.00
    • National Institutes of Health: $357,750.00
    • National Institutes of Health
    • National Institutes of Health
    • National Institutes of Health
    • National Institutes of Health: $398,920.00
    • National Institutes of Health: $375,514.00
    • National Institutes of Health: $379,816.00
    • National Institutes of Health: $525,975.00
    • National Institutes of Health: $357,750.00
    • National Institutes of Health
    • National Institutes of Health: $357,750.00
    • National Institutes of Health

    ASJC

    • Medicine(all)

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