GENETIC ANALYSIS OF MYOGENIC CELL DETERMINATION

    Project: Research project

    Description

    A major effort in contemporary biological research is to identify loci
    that control developmental decisions. In addition, disturbance of the
    normal differentiation process can contribute to tumorigenesis.
    Determining the key regulatory mechanisms involved in generating and
    maintaining the differentiated state is important for understanding both
    normal development and disease. This is a proposal to investigate the control of cell type determination
    in mammalian cells. By utilizing myoblast cell lines grown in vitro, and
    a combined somatic cell and molecular genetic approach, the regulation of
    the MyoD family of myogenic regulatory genes will be analyzed.
    Intertypic hybrids will be assayed for activation and repression of
    myogenesis. Genetic studies of this nature have been utilized to
    identify positive and negative regulatory interactions in a number of
    experimental systems. This project has four goals: 1) define the role of negative regulation of
    MyoD in 10T1/2 X fibroblast hybrids, 2) determine whether additional
    fibroblast chromosomes activate muscle, fat, or cartilage phenotypes in
    1OT1/2 cells, 3) determine whether MyoD is repressed in cis or in trans
    in 1OT1/2 cells, and 4) examine the genetic basis of extinction in 1OT1/2
    X L6 myoblast hybrids. By identifying and ultimately isolating these
    unique regulators, we can begin to define the genetic mechanisms involved
    in the determination and differentiation of muscle cells. An
    understanding of the regulatory circuits which control the myogenic
    lineage will give important insights into the perturbations of gene
    expression that result in abnormal muscle development and tumorigenesis.
    StatusFinished
    Effective start/end date5/1/934/30/02

    Funding

    • National Institutes of Health: $240,799.00
    • National Institutes of Health
    • National Institutes of Health
    • National Institutes of Health
    • National Institutes of Health
    • National Institutes of Health
    • National Institutes of Health: $233,785.00
    • National Institutes of Health
    • National Institutes of Health

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    Myoblasts
    Muscle Development
    Carcinogenesis
    Molecular Biology
    Muscles
    Regulator Genes
    Rhabdomyosarcoma
    Internal-External Control
    Cell Line
    Isochromosomes
    Fibroblasts
    Aptitude
    Genetic Loci
    Gene Duplication
    Muscle Cells
    Gene Amplification
    Cartilage
    Research
    Chromosomes
    Matrix Metalloproteinase 3

    ASJC

    • Medicine(all)
    • Biochemistry, Genetics and Molecular Biology(all)