FOUR DIMENSIONAL IMAGING OF THE IMMUNE RESPONSE IN VIVO

Project: Research project

Project Details

Description

New technologies have allowed us to image the immune response in vivo in 3 spatial dimensions as well as time. The rodent eye is ideal for in vivo imaging of the immune response. The eye is a well recognized target of immune-mediated inflammation known as uveitis. We have succeeded in imaging leukocyte-endothelial interactions in the inflamed mouse and rat eye in a system that involves no surgical trauma and which allows repetitive observation over many days. Using the technique of ocular intravital microscopy, we propose to: 1) determine the role of antigen specific T cells in immune-mediated or autoimmune inflammation; 2) determine the role of specific adhesion molecules or cytokines in an in vivo model of immune-mediated inflammation; 3) identify the site and kinetics of T cell activation in vivo in a disease model; and 4) enhance the technology that we are currently using to obtain 4 dimensional imaging of the immune response. These studies offer a unique opportunity to develop modalities to image the immune response in vivo.
StatusFinished
Effective start/end date9/30/991/31/12

Funding

  • National Institutes of Health: $591,096.00
  • National Institutes of Health: $528,500.00
  • National Institutes of Health
  • National Institutes of Health: $528,500.00
  • National Institutes of Health: $575,364.00
  • National Institutes of Health: $547,122.00
  • National Institutes of Health: $528,500.00
  • National Institutes of Health: $528,500.00
  • National Institutes of Health: $569,901.00
  • National Institutes of Health: $617,744.00

ASJC

  • Medicine(all)

Fingerprint Explore the research topics touched on by this project. These labels are generated based on the underlying awards/grants. Together they form a unique fingerprint.