Effects of autoantibodies on synaptic transmission in the retina

    Project: Research project

    Description

    DESCRIPTION (provided by applicant): Melanoma Associated Retinopathy (MAR) is a paraneoplastic visual syndrome associated with cutaneous malignant melanoma in which patients typically experience a sudden decrease in night vision and sensations of shimmering lights. Electroretinogram (ERG) recordings from MAR patients are characterized by a negative waveform in which the a-wave, arising from photoreceptor transduction, is normal, but the b-wave, arising primarily from ON-bipolar cell activation, is absent or reduced. Significantly, serum
    from MAR patients has been shown to label retinal bipolar cells. The proposed research tests the hypothesis that autoantibodies in MAR patient serum are generated against proteins expressed in metastatic malignant melanocytes, but then target a protein complex functional in bipolar cells. The aims of the research are to identify the retinal antigens in MAR, to elucidate the role of these proteins in normal bipolar cell physiology, and determine how these functions are perturbed in MAR. The aims will be addressed with a multidisciplinary approach combining immunohistochemistry, cell biology, biochemistry, and electrophysiology. The results will generate new insights into cellular processes fundamental to vision and will contribute to our understanding of the link between cancer and autoimmune retinopathy.
    StatusFinished
    Effective start/end date4/1/134/30/17

    Funding

    • National Institutes of Health: $462,428.00
    • National Institutes of Health: $462,428.00
    • National Institutes of Health: $471,865.00
    • National Institutes of Health: $471,865.00

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    Ocular Paraneoplastic Syndromes
    Synaptic Transmission
    Autoantibodies
    Retina
    Retinal Bipolar Cells
    Night Vision
    Paraneoplastic Syndromes
    Cell Physiological Phenomena
    Proteins
    Electrophysiology
    Melanocytes
    Research
    Biochemistry
    Cell Biology
    Immunohistochemistry
    Light
    Antigens
    Serum

    ASJC

    • Medicine(all)