ECTOPIC HORMONE SYNTHESIS IN PHEOCHROMOCYTOMA CELLS

Project: Research project

Description

The applicant's long-term objective is to elucidate the pathways
that determine the pattern of neuropeptide synthesis in neural
crest-derived tumors. Expression of vasoactive intestinal
polypeptide (VIP) in pheochromocytoma and neuroblastoma cells is
regulated by cAMP and protein kinase C-dependent pathways.
The two questions that will be addressed are 1) How do second
messenger systems regulate expression of the VIP gene and 2) Why
is expression of the VIP gene restricted to neural crest-derived
cells? During the first grant period, a 25 base pair element within
the promotor region of the VIP gene was identified, which is
required for stimulation of VIP synthesis of cAMP. The specific
aims of the current proposal are to define the structural
requirements for the cAMP-responsive element, to determine
whether the element is an enhancer, a promoter, or some other
kind of regulatory sequence, to characterize specific proteins that
bind to the element, to determine whether the cAMP-responsive
element mediates the effects of other second messenger systems,
and to determine whether there are cis-acting elements within
the VIP gene that permit expression only in neural crest-derived
tissues. Understanding the mechanisms by which classical second
messengers and other trans-acting factors regulate VIP production
may help explain why some neural crest-derived tumors produce
VIP.
StatusFinished
Effective start/end date3/1/843/31/92

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $166,336.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

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Ectopic Hormones
Pheochromocytoma
Neural Crest
Genes
Trans-Activators
Organized Financing
Neuropeptides
Vasoactive Intestinal Peptide
Second Messenger Systems
Cyclic AMP-Dependent Protein Kinases
Neuroblastoma
Genetic Promoter Regions
Base Pairing
Protein Kinase C
Gene Expression
Neoplasms
Proteins
Nerve Growth Factor

ASJC

  • Medicine(all)