DENDRITIC TRANSPORT

  • Banker, Gary (PI)

Project: Research project

Project Details

Description

The form and physiology of the dendrites of nerve cells determine many
aspects of neuronal function. The intradendritic transport of materials
synthesized in the cell body must play a prominent role in controlling the
formation of dendrites during development, in regulating their potential
for modification in response to neuronal activity, and in determining their
capacity for reinnervation following neural injury. The lack of direct
information concerning the transport of materials from cell body to
dendrites represents a major gap in our understanding of these crucial
events. In the present proposal dendritic transport will be studied in hippocampal
nerve cells maintained in culture by administering 3H uridine, which is
incorporated exclusively in the cell body, and monitoring the transport of
radioactive RNA into the dendrites by light microscopic autoradiography.
In order to determine the rate of migration and to establish whether
transport is an active process, the distance of transport will be assessed
at varying times after labeling and the effects of inhibitors of axonal
transport will be assessed. In order to address the mechanism of RNA
transport in dendrites, several studies will investigate the relationship
between RNA and the dendritic cytoskeleton. The association of dendritic
mRNA and rRNA with detergent-extracted cytoskeletal preparations will be
assessed using in situ hybridization, both in normal cells and after
treatment with agents that dissociate polyribosomes. Binding between the
cytoskeleton and RNA which is in transit within the dendrites will be
assessed following labeling with 3H uridine, and the effects of
dissociation of polyribosomes on transport of mRNA and rRNA will be
assessed. To determine whether other molecules are transported within
dendrites at similar rates, the transport of glycoproteins will be studied
following labeling with precursor sugars. Finally, since innervation
regulates many dendritic properties, the influence of innervation will be
assessed by measuring transport rates in cultures established at both high
and low plating densitites, conditions which either permit extensive
synaptogenesis or greatly limit the possibilities for synaptic interaction.
StatusFinished
Effective start/end date1/1/866/30/01

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $176,639.00
  • National Institutes of Health: $192,663.00
  • National Institutes of Health
  • National Institutes of Health: $159,321.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $284,633.00
  • National Institutes of Health

ASJC

  • Medicine(all)
  • Neuroscience(all)

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