• Heffron, Fred (PI)

    Project: Research project

    Project Details


    The projects described by the users in this application share the common
    approach of using a combination of cell biology and quantitative optical
    techniques to explore a variety of processes. These investigators are most
    frequently dependent on the resolution of fluorescent indicators in living
    cells. The instrument chosen is a Leica CLSM confocal laser scan with a
    Fluorvert inverted microscope that will be 50% cost shared with the
    university. The design includes a complete two-channel detector system
    that allows simultaneous measurements of two different fluorescent labels
    in precise register for all image points. The high focusing accuracy
    provided will enable more precise measurements of cell volume than that
    afforded by other instruments and the Leica images are the best that we
    have observed. All of the users in this application have been funded by
    NIH for their work and for the experiments described in this proposal. The
    confocal microscope will bring a new dimension to their research by
    providing better resolution, removing uncertainty about co-localization,
    making measurements easier and in some cases, possible, for the first time.
    Several different types of measurements are described. In some cases the
    investigators will be exploring new territory in trying to make sense of
    a complicated pathogenesis pathway. In Forte's case, the ability to
    optically section entire Drosophila embryo is the best way to follow the
    developmental regulation of specific neural components. The Westbrook
    experiments will test a specific model for activation of a neural receptor
    by making simultaneous measurements of intracellular calcium levels and the
    activation state of the receptor -- difficult measurements to make without
    the resolving power of the confocal. Low's group currently makes many
    measurements using traditional fluorescence to compare co-localization of
    specific stains. The same measurements that take all day can be made more
    accurately using optical sectioning and the data can be stored on disk in
    just minutes. Thornberg's group need to make cell volume measurements that
    cannot be achieved in any other way than with a confocal.
    Effective start/end date5/5/935/4/94


    • National Institutes of Health


    • Medicine(all)


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