CLONING OF THE CATECHOLAMINE UPTAKE TRANSPORTER

Project: Research project

Description

The long term goals are to study the regulation of neuronal transmission in
the central nervous system and to understand the role of pharmacological
agents in modifying neuronal transmission. The current proposal to clone
the catecholamine uptake transmitter reflects to the application of basic
science to clinical issues . The importance of catecholamine uptake
transporters is demonstrated by the impact in our society of specific
inhibitors of these proteins, namely cocaine, amphetamines, and tri-cyclic
antidepressants. 'Me stimulatory action and abuse potential is directly
related to their ability to bind and block catecholamine uptake at the
presynaptic nerve terminals. To study the pharmacology of cocaine and to understand the molecular basis
for its medical effects, it is necessary to isolate and characterize the
transporter proteins themselves. Molecular cloning of catecholamine
transporters is the most direct way to gain access to this class of
transporters. Their ability to bind inhibitors with high affinity and to
concentrate catecholamines within a cell make these proteins ideally suited
to molecular cloning by expression in heterologous cells. This application
presents a cloning strategy employing radiolabelled ligands and substrates
to isolate the cDNA encoding a catecholamine transporter following its
expressing in COS cells.
StatusFinished
Effective start/end date4/1/913/31/96

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

Fingerprint

Catecholamines
Organism Cloning
Molecular Cloning
Cocaine
Amphetamines
Proteins
COS Cells
Action Potentials
Pharmacology
Central Nervous System
Complementary DNA
Ligands

ASJC

  • Medicine(all)