CHARACTERIZATION AND FUNCTION OF THE GNRH:SH GENE LOCUS

  • Adelman, John (PI)

Project: Research project

Project Details

Description

The function of the Gonadotropin Releasing Hormone (GnRH) gene is
central to the reproductive capability of mammalian organisms. At
the cellular level many factors may act to regulate the production
of the GnRH decapeptide via effects on transcription, translation
and post-translational events. The structures of the mRNA and
genomic locus encoding the GnRH precursor in the rat and human have
been described. Further work revealed that the DNA in the rat
which encodes the GnRH precursor on one DNA strand, also encodes
a distinct gene, SH, on the opposite DNA strand. The SH and GnRH
genes share significance exonic sequences and the RNAs transcribed
from either strand show distinct but overlapping patterns of tissue
and cellular specificity. The major goal of the research proposed here is to more thoroughly
characterize the GnRH:SH gene locus and examine the functional
relationship between the GnRH and SH gene products. First, will
be to localize the promoter elements present on both strands of the
GnRH:SH gene by mapping transcriptional initiation sites, and by
assaying the ability of implicated genomic sequences to drive
expression of reporter genes. Second, will be to determine if the
SH RNAs produce proteins in vivo. Third, will be to determine
whether there are cells which co-express the GnRH and SH genes.
Fourth, will be to examine the consequences of expressing the GnRH
and SH genes within the same cells. By studying the structure and function of the GnRH:SH gene locus
in this way, we hope to answer questions regarding the regulation
of the reproductive control molecule GnRH, the function of the SH
gene, and the significance of this novel eukaryotic genetic
arrangement.
StatusFinished
Effective start/end date12/1/8811/30/91

Funding

  • National Institutes of Health
  • National Institutes of Health: $147,833.00
  • National Institutes of Health

ASJC

  • Medicine(all)

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