B LYMPHOCYTE ACTIVATION

  • Parker, David, (PI)

Project: Research project

Description

The investigators will study the role of membrane Ig and the
function of helper T cells in the antibody response by exploiting a
new, murine, in vitro polyclonal model for T cell/B cell
cooperation. In this model, rabbit antibody against mouse
immunoglobulin (anti-Ig) is used in place of antigen, and T cell
help is provided by cultured T cell lines and cloned T cell
hybridomas which recognize the rabbit anti-Ig reagents as foreign
protein antigens. The investigators have shown that normal, small
B cells act as extraordinarily efficient antigen presenting cells for
rabbit anti-IgM or anti-IgD. Recognition of anti-Ig on the B cell
surface is restricted by class II products of the major
histocompatibility complex (MHC), and results in both a T cell
response and a vigorous polyclonal B cell response. The model will be used to define the role of membrane Ig in
antigen processing (if processing occurs in small B cells) and
presentation of specifically bound antigen to helper T cells.
Functional heterogeneity of helper T cells in their ability to
interact with small B cells or other antigen presenting cells will
be defined and explored. The mechanism of delivery of T cell
help will be studied to determine whether help can be accounted
for by secretion of characterized or novel lymphokines, or
requires an early, contact-mediated event involving stimulated
secretion of preformed mediators or interaction of membrane-
bound molecules other then those required for specific recognition
by the T cell. Finally, in an attempt to relate immune cell physiology to the
cellular machinery which regulates cell division, the investigators
have begun to study the effects of agents which activate B
lymphocytes to different extents or by different pathways on the
levels of cmyc mRNA in the cell during the first hours of the
activation sequence. These studies will be extended to other
cellular protooncogenes (c-fos, c-myb, c-ras) and genes induced
by growth signals in fibroblasts (JE and KC), and transcription
rates will be compared with mRNA levels.
StatusFinished
Effective start/end date7/1/8711/30/98

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $134,765.00
  • National Institutes of Health: $219,396.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

Fingerprint

B-Lymphocytes
Helper-Inducer T-Lymphocytes
T-Lymphocytes
Antigen-Presenting Cells
CD40 Ligand
Membranes
Antigens
Lymphokines
Research Personnel
Rabbits
Cell Physiological Phenomena
Messenger RNA
ras Genes
Viral Tumor Antigens
Antibody Formation
Cell Division
Cultured Cells
Fibroblasts
Antibodies
Cell Line

ASJC

  • Medicine(all)
  • Immunology and Microbiology(all)