Ninian Blackburn

Professor

  • 5140 Citations
  • 46 h-Index
1975 …2020
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Research Output 1975 2019

  • 5140 Citations
  • 46 h-Index
  • 115 Article
  • 1 Chapter
  • 1 Conference contribution
2019

Rational Design of a Histidine-Methionine Site Modeling the M-Center of Copper Monooxygenases in a Small Metallochaperone Scaffold

Alwan, K. B., Welch, E. F., Arias, R. J., Gambill, B. F. & Blackburn, N., Jan 1 2019, In : Biochemistry.

Research output: Contribution to journalArticle

Metallochaperones
Mixed Function Oxygenases
Histidine
Scaffolds
Methionine
2017
15 Citations (Scopus)

Copper-zinc superoxide dismutase is activated through a sulfenic acid intermediate at a copper ion entry site

Fetherolf, M. M., Boyd, S. D., Taylor, A. B., Kim, H. J., Wohlschlegel, J. A., Blackburn, N., Hart, P. J., Winge, D. R. & Winkler, D. D., 2017, In : Journal of Biological Chemistry. 292, 29, p. 12025-12040 16 p.

Research output: Contribution to journalArticle

Sulfenic Acids
Superoxide Dismutase
Zinc
Copper
Ions
7 Citations (Scopus)

Effect of circular permutation on the structure and function of type 1 blue copper center in azurin

Yu, Y., Petrik, I. D., Chacón, K. N., Hosseinzadeh, P., Chen, H., Blackburn, N. & Lu, Y., Feb 1 2017, In : Protein Science. 26, 2, p. 218-226 9 p.

Research output: Contribution to journalArticle

Azurin
Copper
Electrons
Proteins
Transfer functions
2016
35 Citations (Scopus)

Design of a single protein that spans the entire 2-V range of physiological redox potentials

Hosseinzadeh, P., Marshall, N. M., Chacón, K. N., Yu, Y., Nilges, M. J., New, S. Y., Tashkov, S. A., Blackburn, N. & Lu, Y., Jan 12 2016, In : Proceedings of the National Academy of Sciences of the United States of America. 113, 2, p. 262-267 6 p.

Research output: Contribution to journalArticle

Oxidation-Reduction
Azurin
Metalloproteins
Surface Properties
Hydrogen
8 Citations (Scopus)

Kβ Valence to Core X-ray Emission Studies of Cu(I) Binding Proteins with Mixed Methionine - Histidine Coordination. Relevance to the Reactivity of the M- and H-sites of Peptidylglycine Monooxygenase

Martin-Diaconescu, V., Chacón, K. N., Delgado-Jaime, M. U., Sokaras, D., Weng, T. C., DeBeer, S. & Blackburn, N., Apr 18 2016, In : Inorganic Chemistry. 55, 7, p. 3431-3439 9 p.

Research output: Contribution to journalArticle

methionine
histidine
Histidine
Methionine
Carrier Proteins
2 Citations (Scopus)

Nano assembly and encapsulation; a versatile platform for slowing the rotation of polyanionic Gd3+-based MRI contrast agents

Farashishiko, A., Chacón, K. N., Blackburn, N. & Woods, M., 2016, (Accepted/In press) In : Contrast Media and Molecular Imaging.

Research output: Contribution to journalArticle

Contrast Media
Capsules
Water Movements
Protons
Water
4 Citations (Scopus)

PH-regulated metal-ligand switching in the HM loop of ATP7A: A new paradigm for metal transfer chemistry

Kline, C. D., Gambill, B. F., Mayfield, M., Lutsenko, S. & Blackburn, N., Aug 1 2016, In : Metallomics. 8, 8, p. 729-733 5 p.

Research output: Contribution to journalArticle

Copper
Metals
Ligands
Histidine
Adenosine Triphosphatases
9 Citations (Scopus)

Reversible S-nitrosylation in an engineered azurin

Tian, S., Liu, J., Cowley, R. E., Hosseinzadeh, P., Marshall, N. M., Yu, Y., Robinson, H., Nilges, M. J., Blackburn, N., Solomon, E. I. & Lu, Y., Jul 1 2016, In : Nature Chemistry. 8, 7, p. 670-677 8 p.

Research output: Contribution to journalArticle

Azurin
Copper
S-Nitrosothiols
Metalloproteins
Proteins
3 Citations (Scopus)

Stopped-Flow Studies of the Reduction of the Copper Centers Suggest a Bifurcated Electron Transfer Pathway in Peptidylglycine Monooxygenase

Chauhan, S., Hosseinzadeh, P., Lu, Y. & Blackburn, N., Apr 19 2016, In : Biochemistry. 55, 13, p. 2008-2021 14 p.

Research output: Contribution to journalArticle

Copper
Electrons
Substrates
Binding Sites
Ligands
3 Citations (Scopus)
Carbon Monoxide
Conformations
Copper
Oxygen
Peptides
2015
7 Citations (Scopus)

Binuclear CuA Formation in Biosynthetic Models of CuA in Azurin Proceeds via a Novel Cu(Cys)2His Mononuclear Copper Intermediate

Chakraborty, S., Polen, M. J., Chacón, K. N., Wilson, T. D., Yu, Y., Reed, J., Nilges, M. J., Blackburn, N. & Lu, Y., Oct 6 2015, In : Biochemistry. 54, 39, p. 6071-6081 11 p.

Research output: Contribution to journalArticle

Azurin
Copper
Electrons
Ligands
Proteins
15 Citations (Scopus)

Multicopper manganese oxidase accessory proteins bind Cu and heme

Butterfield, C. N., Tao, L., Chacón, K. N., Spiro, T. G., Blackburn, N., Casey, W. H., Britt, R. D. & Tebo, B., Dec 1 2015, In : Biochimica et Biophysica Acta - Proteins and Proteomics. 1854, 12, p. 1853-1859 7 p.

Research output: Contribution to journalArticle

Accessories
Manganese
Heme
Oxidoreductases
Electrons
2014
8 Citations (Scopus)

Binding of copper and silver to single-site variants of peptidylglycine monooxygenase reveals the structure and chemistry of the individual metal centers

Chauhan, S., Kline, C. D., Mayfield, M. & Blackburn, N., Feb 18 2014, In : Biochemistry. 53, 6, p. 1069-1080 12 p.

Research output: Contribution to journalArticle

Silver
Copper
Metals
X ray absorption
Carbon Monoxide
4 Citations (Scopus)

Copper in eukaryotes

Blackburn, N., Yan, N. & Lutsenko, S., 2014, Binding, Transport and Storage of Metal Ions in Biological Cells. 2 ed. Royal Society of Chemistry, Vol. 2014-January. p. 524-555 32 p. (RSC Metallobiology; vol. 2014-January, no. 2).

Research output: Chapter in Book/Report/Conference proceedingChapter

Eukaryota
Copper
13 Citations (Scopus)

Copper-peptide complex structure and reactivity when found in conserved His-Xaa-His sequences

Park, G. Y., Lee, J. Y., Himes, R. A., Thomas, G. S., Blackburn, N. & Karlin, K. D., Sep 10 2014, In : Journal of the American Chemical Society. 136, 36, p. 12532-12535 4 p.

Research output: Contribution to journalArticle

Peptides
Copper
Carbon Monoxide
X-Ray Absorption Spectroscopy
X ray absorption spectroscopy
11 Citations (Scopus)

Modulating the copper-sulfur interaction in type 1 blue copper azurin by replacing Cys112 with nonproteinogenic homocysteine

Clark, K. M., Yu, Y., Van Der Donk, W. A., Blackburn, N. & Lu, Y., Feb 1 2014, In : Inorganic Chemistry Frontiers. 1, 2, p. 153-158 6 p.

Research output: Contribution to journalArticle

Azurin
Homocysteine
Sulfur
Copper
Amino Acids
26 Citations (Scopus)

Tracking metal ions through a Cu/Ag efflux pump assigns the functional roles of the periplasmic proteins

Chacón, K. N., Mealman, T. D., McEvoy, M. M. & Blackburn, N., Oct 28 2014, In : Proceedings of the National Academy of Sciences of the United States of America. 111, 43, p. 15373-15378 6 p.

Research output: Contribution to journalArticle

Periplasmic Proteins
Copper
Metals
Ions
Periplasm
2013
12 Citations (Scopus)

HHM motif at the CuH-Site of peptidylglycine monooxygenase is a pH-dependent conformational switch

Kline, C. D., Mayfield, M. & Blackburn, N., Apr 16 2013, In : Biochemistry. 52, 15, p. 2586-2596 11 p.

Research output: Contribution to journalArticle

Copper
Switches
Sulfides
Catalyst activity
Nitrogen
10 Citations (Scopus)

Interdomain long-range electron transfer becomes rate-limiting in the Y216A variant of tyramine β-monooxygenase

Osborne, R. L., Zhu, H., Iavarone, A. T., Blackburn, N. & Klinman, J. P., Feb 19 2013, In : Biochemistry. 52, 7, p. 1179-1191 13 p.

Research output: Contribution to journalArticle

Tyramine
Mixed Function Oxygenases
Electrons
Copper
Substrates
2012
20 Citations (Scopus)

Alternative ground states enable pathway switching in biological electron transfer

Abriata, L. A., Álvarez-Paggi, D., Ledesma, G. N., Blackburn, N., Vila, A. J. & Murgida, D. H., Oct 23 2012, In : Proceedings of the National Academy of Sciences of the United States of America. 109, 43, p. 17348-17353 6 p.

Research output: Contribution to journalArticle

Electrons
Proteins
Cell Respiration
Thermus thermophilus
Biological Phenomena
13 Citations (Scopus)

Lumenal loop M672-P707 of the menkes protein (ATP7A) transfers copper to peptidylglycine monooxygenase

Otoikhian, A., Barry, A. N., Mayfield, M., Nilges, M., Huang, Y., Lutsenko, S. & Blackburn, N., Jun 27 2012, In : Journal of the American Chemical Society. 134, 25, p. 10458-10468 11 p.

Research output: Contribution to journalArticle

Copper
Proteins
Adenosine Triphosphatases
peptidylglycine monooxygenase
X-Ray Absorption Spectroscopy
18 Citations (Scopus)

Metal Export by CusCFBA, the Periplasmic Cu(I)/Ag(I) Transport System of Escherichia coli

Mealman, T. D., Blackburn, N. & McEvoy, M. M., 2012, In : Current Topics in Membranes. 69, p. 163-196 34 p.

Research output: Contribution to journalArticle

Metals
Escherichia coli
Ions
Metallochaperones
Periplasm
21 Citations (Scopus)

N-Terminal region of CusB is sufficient for metal binding and metal transfer with the metallochaperone CusF

Mealman, T. D., Zhou, M., Affandi, T., Chacón, K. N., Aranguren, M. E., Blackburn, N., Wysocki, V. H. & McEvoy, M. M., Aug 28 2012, In : Biochemistry. 51, 34, p. 6767-6775 9 p.

Research output: Contribution to journalArticle

Metallochaperones
Metals
Membrane Fusion Proteins
Cell Division
Escherichia coli
21 Citations (Scopus)
Thermus thermophilus
Electron Transport Complex IV
Copper
Azurin
Selenomethionine
2011
18 Citations (Scopus)

A copper-methionine interaction controls the pH-dependent activation of peptidylglycine monooxygenase

Bauman, A. T., Broers, B. A., Kline, C. D. & Blackburn, N., Dec 20 2011, In : Biochemistry. 50, 50, p. 10819-10828 10 p.

Research output: Contribution to journalArticle

Methionine
Copper
Catalyst activity
Chemical activation
X ray absorption
22 Citations (Scopus)
Electron Transport Complex IV
Bacilli
Bacillus subtilis
Selenomethionine
Copper
27 Citations (Scopus)

The lumenal loop Met 672-Pro 707 of copper-transporting ATPase ATP7A binds metals and facilitates copper release from the intramembrane sites

Barry, A. N., Otoikhian, A., Bhatt, S., Shinde, U., Tsivkovskii, R., Blackburn, N. & Lutsenko, S., Jul 29 2011, In : Journal of Biological Chemistry. 286, 30, p. 26585-26594 10 p.

Research output: Contribution to journalArticle

Copper
Metals
histidylhistidine
Secretory Pathway
alanylglycine
2010
29 Citations (Scopus)

Anatomy of a red copper center: Spectroscopic identification and reactivity of the copper centers of bacillus subtilis Sco and its Cys-to-ala variants

Siluvai, G. S., Mayfield, M., Nilges, M. J., Debeer George, S. & Blackburn, N., Apr 14 2010, In : Journal of the American Chemical Society. 132, 14, p. 5215-5226 12 p.

Research output: Contribution to journalArticle

Bacilli
Bacillus subtilis
Copper
Anatomy
Proteins
5 Citations (Scopus)

A Tale of Two Metals

Blackburn, N., Jan 29 2010, In : Chemistry and Biology. 17, 1, p. 8-9 2 p.

Research output: Contribution to journalArticle

Protein Transport
Copper
Metals
Proteins
tetrathiomolybdate
24 Citations (Scopus)

Interactions between copper-binding sites determine the redox status and conformation of the regulatory N-terminal domain of ATP7B

LeShane, E. S., Shinde, U., Walker, J. M., Barry, A. N., Blackburn, N., Ralle, M. & Lutsenko, S., Feb 26 2010, In : Journal of Biological Chemistry. 285, 9, p. 6327-6336 10 p.

Research output: Contribution to journalArticle

Oxidation-Reduction
Conformations
Copper
Metals
Binding Sites
17 Citations (Scopus)

The copper centers of tyramine β-monooxygenase and its catalytic-site methionine variants: An X-ray absorption study

Hess, C. R., Klinman, J. P. & Blackburn, N., Nov 2010, In : Journal of Biological Inorganic Chemistry. 15, 8, p. 1195-1207 13 p.

Research output: Contribution to journalArticle

Tyramine
X ray absorption
Mixed Function Oxygenases
X-Ray Absorption Spectroscopy
Methionine
43 Citations (Scopus)

Transforming a blue copper into a red copper protein: Engineering cysteine and homocysteine into the axial position of azurin using site-directed mutagenesis and expressed protein ligation

Clark, K. M., Yu, Y., Marshall, N. M., Sieracki, N. A., Nilges, M. J., Blackburn, N., Van Der Donk, W. A. & Lu, Y., Jul 28 2010, In : Journal of the American Chemical Society. 132, 29, p. 10093-10101 9 p.

Research output: Contribution to journalArticle

Azurin
Protein Engineering
Mutagenesis
Homocysteine
Site-Directed Mutagenesis
2009
23 Citations (Scopus)

H135A controls the redox activity of the Sco copper center. Kinetic and spectroscopic studies of the His135Ala variant of Bacillus subtilis Sco

Siluvai, G. S., Nakano, M., Mayfield, M., Nilges, M. J. & Blackburn, N., Dec 29 2009, In : Biochemistry. 48, 51, p. 12133-12144 12 p.

Research output: Contribution to journalArticle

Bacilli
Bacillus subtilis
Oxidation-Reduction
Copper
Kinetics
174 Citations (Scopus)

Three-dimensional structure of the human copper transporter hCTR1

De Feo, C. J., Aller, S. G., Siluvai, G. S., Blackburn, N. & Unger, V. M., Mar 17 2009, In : Proceedings of the National Academy of Sciences of the United States of America. 106, 11, p. 4237-4242 6 p.

Research output: Contribution to journalArticle

Copper
Metals
Binding Sites
Crystallography
Eukaryota
24 Citations (Scopus)

Tryptophan Cu(I)-π interaction fine-tunes the metal binding properties of the bacterial metallochaperone CusF

Loftin, I. R., Blackburn, N. & McEvoy, M. M., 2009, In : Journal of Biological Inorganic Chemistry. 14, 6, p. 905-912 8 p.

Research output: Contribution to journalArticle

Metallochaperones
Tryptophan
Metals
Ligands
X ray absorption spectroscopy
2008
14 Citations (Scopus)
Selenocysteine
Dimers
Superoxide Dismutase
Copper
Chemical analysis
78 Citations (Scopus)

Direct metal transfer between periplasmic proteins identifies a bacterial copper chaperone

Bagai, I., Rensing, C., Blackburn, N. & McEvoy, M. M., Nov 4 2008, In : Biochemistry. 47, 44, p. 11408-11414 7 p.

Research output: Contribution to journalArticle

Periplasmic Proteins
Copper
Metals
Periplasm
Gram-Negative Bacteria
12 Citations (Scopus)

Selenocysteine positional variants reveal contributions to copper binding from cysteine residues in domains 2 and 3 of human copper chaperone for superoxide dismutase

Barry, A. N., Clark, K. M., Otoikhian, A., Van Der Donk, W. A. & Blackburn, N., Dec 9 2008, In : Biochemistry. 47, 49, p. 13074-13083 10 p.

Research output: Contribution to journalArticle

Selenocysteine
Superoxide Dismutase
Cysteine
Copper
Derivatives
104 Citations (Scopus)

Structural studies of copper(I) complexes of amyloid-β peptide fragments: Formation of two-coordinate bis(histidine) complexes

Himes, R. A., Park, G. Y., Siluvai, G. S., Blackburn, N. & Karlin, K. D., Nov 10 2008, In : Angewandte Chemie - International Edition. 47, 47, p. 9084-9087 4 p.

Research output: Contribution to journalArticle

Peptide Fragments
Histidine
Amyloid
Peptides
Copper
2007
40 Citations (Scopus)

A multinuclear copper(I) cluster forms the dimerization interface in copper-loaded human copper chaperone for superoxide dismutase

Stasser, J. P., Siluvai, G. S., Barry, A. N. & Blackburn, N., Oct 23 2007, In : Biochemistry. 46, 42, p. 11845-11856 12 p.

Research output: Contribution to journalArticle

Dimerization
Dimers
Superoxide Dismutase
Copper
Oligomerization
8 Citations (Scopus)

Large scale production of the copper enzyme peptidylglycine monooxygenase using an automated bioreactor

Bauman, A. T., Ralle, M. & Blackburn, N., Jan 2007, In : Protein Expression and Purification. 51, 1, p. 34-38 5 p.

Research output: Contribution to journalArticle

Bioreactors
Rats
Copper
Biochemistry
CHO Cells
63 Citations (Scopus)

Substrate-linked conformational change in the periplasmic component of a Cu(I)/Ag(I) efflux system

Bagai, I., Liu, W., Rensing, C., Blackburn, N. & McEvoy, M. M., Dec 7 2007, In : Journal of Biological Chemistry. 282, 49, p. 35695-35702 8 p.

Research output: Contribution to journalArticle

Metals
Substrates
Methionine
Copper
X-Ray Absorption Spectroscopy
76 Citations (Scopus)

Synthesis and x-ray absorption spectroscopy structural studies of Cu(I) complexes of HistidylHistidine peptides: The predominance of linear 2-coordinate geometry

Himes, R. A., Ga, Y. P., Barry, A. N., Blackburn, N. & Karlin, K. D., May 2 2007, In : Journal of the American Chemical Society. 129, 17, p. 5352-5353 2 p.

Research output: Contribution to journalArticle

histidylhistidine
Absorption spectroscopy
Peptides
Copper
Spectrum Analysis
89 Citations (Scopus)

Unusual Cu(I)/Ag(I) coordination of Escherichia coli CusF as revealed by atomic resolution crystallography and X-ray absorption spectroscopy

Loftin, I. R., Franke, S., Blackburn, N. & Mcevoy, M. M., Oct 2007, In : Protein Science. 16, 10, p. 2287-2293 7 p.

Research output: Contribution to journalArticle

X-Ray Absorption Spectroscopy
Crystallography
X ray absorption spectroscopy
Silver
Escherichia coli
6 Citations (Scopus)

Using XAS and SXRF to study copper in wilson disease at the molecular and tissue level

Ralle, M., Blackburn, N. & Lutsenko, S., 2007, AIP Conference Proceedings. Vol. 882. p. 328-330 3 p.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

synchrotrons
copper
fluorescence
x rays
liver
2006
18 Citations (Scopus)

pH dependence of peptidylglycine monooxygenase. Mechanistic implications of Cu-methionine binding dynamics

Bauman, A. T., Jaron, S., Yukl, E. T., Burchfiel, J. R. & Blackburn, N., Sep 19 2006, In : Biochemistry. 45, 37, p. 11140-11150 11 p.

Research output: Contribution to journalArticle

Methionine
Buffers
Sulfonic Acids
Acetates
Deprotonation
17 Citations (Scopus)

Role for an essential tyrosine in peptide amidation

De, M., Bell, J., Blackburn, N., Mains, R. E. & Eipper, B. A., Jul 28 2006, In : Journal of Biological Chemistry. 281, 30, p. 20873-20882 10 p.

Research output: Contribution to journalArticle

Lyases
Tyrosine
Catalytic Domain
Iron
Peptides
30 Citations (Scopus)

The catalytic role of the copper ligand H172 of peptidylglycine α-hydroxylating monooxygenase: A kinetic study of the H172A mutant

Evans, J. P., Blackburn, N. & Klinman, J. P., Dec 26 2006, In : Biochemistry. 45, 51, p. 15419-15429 11 p.

Research output: Contribution to journalArticle

Mixed Function Oxygenases
Isotopes
Copper
Electrons
Ligands
42 Citations (Scopus)

The hydrogen peroxide reactivity of peptidylglycine monooxygenase supports a Cu(II)-superoxo catalytic intermediate

Bauman, A. T., Yukl, E. T., Alkevich, K., McCormack, A. L. & Blackburn, N., Feb 17 2006, In : Journal of Biological Chemistry. 281, 7, p. 4190-4198 9 p.

Research output: Contribution to journalArticle

Peroxides
Hydrogen Peroxide
Oxygen
Labels
Glucose Oxidase
2005
23 Citations (Scopus)

Cysteine-to-serine mutants of the human copper chaperone for superoxide dismutase reveal a copper cluster at a domain III dimer interface

Stasser, J. P., Eisses, J. F., Barry, A. N., Kaplan, J. H. & Blackburn, N., Mar 8 2005, In : Biochemistry. 44, 9, p. 3143-3152 10 p.

Research output: Contribution to journalArticle

Dimers
Serine
Superoxide Dismutase
Cysteine
Copper