Ninian Blackburn

Professor

  • 5417 Citations
  • 47 h-Index
1975 …2019

Research output per year

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Research Output

2019

Incorporation of Ni2+, Co2+, and Selenocysteine into the Auxiliary Fe-S Cluster of the Radical SAM Enzyme HydG

Rao, G., Alwan, K. B., Blackburn, N. J. & Britt, R. D., Oct 7 2019, In : Inorganic Chemistry. 58, 19, p. 12601-12608 8 p.

Research output: Contribution to journalArticle

1 Scopus citations

PH-Induced Binding of the Axial Ligand in an Engineered CuA Site Favors the πu State

Morgada, M. N., Emiliani, F., Chacón, K. N., Álvarez-Paggi, D., Murgida, D. H., Blackburn, N. J., Abriata, L. A. & Vila, A. J., Dec 2 2019, In : Inorganic Chemistry. 58, 23, p. 15687-15691 5 p.

Research output: Contribution to journalArticle

Rational Design of a Histidine-Methionine Site Modeling the M-Center of Copper Monooxygenases in a Small Metallochaperone Scaffold

Alwan, K. B., Welch, E. F., Arias, R. J., Gambill, B. F. & Blackburn, N. J., Jun 20 2019, In : Biochemistry. 58, 28, p. 3097-3108 12 p.

Research output: Contribution to journalArticle

2 Scopus citations

The binuclear cluster of [FeFe] hydrogenase is formed with sulfur donated by cysteine of an [Fe(Cys)(CO)2(CN)] organometallic precursor

Rao, G., Pattenaude, S. A., Alwan, K., Blackburn, N. J., Britt, R. D. & Rauchfuss, T. B., Oct 15 2019, In : Proceedings of the National Academy of Sciences of the United States of America. 116, 42, p. 20850-20855 6 p.

Research output: Contribution to journalArticle

Open Access
5 Scopus citations
2018

Effects of copper occupancy on the conformational landscape of peptidylglycine α-hydroxylating monooxygenase

Maheshwari, S., Shimokawa, C., Rudzka, K., Kline, C. D., Eipper, B. A., Mains, R. E., Gabelli, S. B., Blackburn, N. & Amzel, L. M., Dec 1 2018, In : Communications Biology. 1, 1, 74.

Research output: Contribution to journalArticle

Open Access
6 Scopus citations

Trapping intermediates in metal transfer reactions of the CusCBAF export pump of Escherichia coli

Chacón, K. N., Perkins, J., Mathe, Z., Alwan, K., Ho, E. N., Ucisik, M. N., Merz, K. M. & Blackburn, N. J., Dec 1 2018, In : Communications Biology. 1, 1, 192.

Research output: Contribution to journalArticle

Open Access
7 Scopus citations
2017

Copper-zinc superoxide dismutase is activated through a sulfenic acid intermediate at a copper ion entry site

Fetherolf, M. M., Boyd, S. D., Taylor, A. B., Kim, H. J., Wohlschlegel, J. A., Blackburn, N., Hart, P. J., Winge, D. R. & Winkler, D. D., 2017, In : Journal of Biological Chemistry. 292, 29, p. 12025-12040 16 p.

Research output: Contribution to journalArticle

25 Scopus citations

Effect of circular permutation on the structure and function of type 1 blue copper center in azurin

Yu, Y., Petrik, I. D., Chacón, K. N., Hosseinzadeh, P., Chen, H., Blackburn, N. J. & Lu, Y., Feb 1 2017, In : Protein Science. 26, 2, p. 218-226 9 p.

Research output: Contribution to journalArticle

9 Scopus citations
2016

Design of a single protein that spans the entire 2-V range of physiological redox potentials

Hosseinzadeh, P., Marshall, N. M., Chacón, K. N., Yu, Y., Nilges, M. J., New, S. Y., Tashkov, S. A., Blackburn, N. J. & Lu, Y., Jan 12 2016, In : Proceedings of the National Academy of Sciences of the United States of America. 113, 2, p. 262-267 6 p.

Research output: Contribution to journalArticle

46 Scopus citations

Kβ Valence to Core X-ray Emission Studies of Cu(I) Binding Proteins with Mixed Methionine - Histidine Coordination. Relevance to the Reactivity of the M- and H-sites of Peptidylglycine Monooxygenase

Martin-Diaconescu, V., Chacón, K. N., Delgado-Jaime, M. U., Sokaras, D., Weng, T. C., DeBeer, S. & Blackburn, N. J., Apr 18 2016, In : Inorganic Chemistry. 55, 7, p. 3431-3439 9 p.

Research output: Contribution to journalArticle

13 Scopus citations

Nano assembly and encapsulation; a versatile platform for slowing the rotation of polyanionic Gd3+-based MRI contrast agents

Farashishiko, A., Chacón, K. N., Blackburn, N. J. & Woods, M., Mar 1 2016, In : Contrast Media and Molecular Imaging. 11, 2, p. 154-159 6 p.

Research output: Contribution to journalArticle

2 Scopus citations

PH-regulated metal-ligand switching in the HM loop of ATP7A: A new paradigm for metal transfer chemistry

Kline, C. D., Gambill, B. F., Mayfield, M., Lutsenko, S. & Blackburn, N. J., Aug 2016, In : Metallomics. 8, 8, p. 729-733 5 p.

Research output: Contribution to journalArticle

6 Scopus citations

Reversible S-nitrosylation in an engineered azurin

Tian, S., Liu, J., Cowley, R. E., Hosseinzadeh, P., Marshall, N. M., Yu, Y., Robinson, H., Nilges, M. J., Blackburn, N. J., Solomon, E. I. & Lu, Y., Jul 1 2016, In : Nature Chemistry. 8, 7, p. 670-677 8 p.

Research output: Contribution to journalArticle

13 Scopus citations

Stopped-Flow Studies of the Reduction of the Copper Centers Suggest a Bifurcated Electron Transfer Pathway in Peptidylglycine Monooxygenase

Chauhan, S., Hosseinzadeh, P., Lu, Y. & Blackburn, N. J., Apr 19 2016, In : Biochemistry. 55, 13, p. 2008-2021 14 p.

Research output: Contribution to journalArticle

7 Scopus citations
6 Scopus citations
2015

Binuclear CuA Formation in Biosynthetic Models of CuA in Azurin Proceeds via a Novel Cu(Cys)2His Mononuclear Copper Intermediate

Chakraborty, S., Polen, M. J., Chacón, K. N., Wilson, T. D., Yu, Y., Reed, J., Nilges, M. J., Blackburn, N. J. & Lu, Y., Oct 6 2015, In : Biochemistry. 54, 39, p. 6071-6081 11 p.

Research output: Contribution to journalArticle

9 Scopus citations

Multicopper manganese oxidase accessory proteins bind Cu and heme

Butterfield, C. N., Tao, L., Chacón, K. N., Spiro, T. G., Blackburn, N. J., Casey, W. H., Britt, R. D. & Tebo, B. M., Dec 1 2015, In : Biochimica et Biophysica Acta - Proteins and Proteomics. 1854, 12, p. 1853-1859 7 p.

Research output: Contribution to journalArticle

16 Scopus citations
2014

Binding of copper and silver to single-site variants of peptidylglycine monooxygenase reveals the structure and chemistry of the individual metal centers

Chauhan, S., Kline, C. D., Mayfield, M. & Blackburn, N. J., Feb 18 2014, In : Biochemistry. 53, 6, p. 1069-1080 12 p.

Research output: Contribution to journalArticle

9 Scopus citations

Copper in eukaryotes

Blackburn, N. J., Yan, N. & Lutsenko, S., Jan 1 2014, In : RSC Metallobiology. 2014-January, 2, p. 524-555 32 p.

Research output: Contribution to journalArticle

4 Scopus citations

Copper-peptide complex structure and reactivity when found in conserved His-Xaa-His sequences

Park, G. Y., Lee, J. Y., Himes, R. A., Thomas, G. S., Blackburn, N. J. & Karlin, K. D., Sep 10 2014, In : Journal of the American Chemical Society. 136, 36, p. 12532-12535 4 p.

Research output: Contribution to journalArticle

16 Scopus citations

Modulating the copper-sulfur interaction in type 1 blue copper azurin by replacing Cys112 with nonproteinogenic homocysteine

Clark, K. M., Yu, Y., Van Der Donk, W. A., Blackburn, N. J. & Lu, Y., Feb 1 2014, In : Inorganic Chemistry Frontiers. 1, 2, p. 153-158 6 p.

Research output: Contribution to journalArticle

12 Scopus citations

Tracking metal ions through a Cu/Ag efflux pump assigns the functional roles of the periplasmic proteins

Chacón, K. N., Mealman, T. D., McEvoy, M. M. & Blackburn, N. J., Oct 28 2014, In : Proceedings of the National Academy of Sciences of the United States of America. 111, 43, p. 15373-15378 6 p.

Research output: Contribution to journalArticle

40 Scopus citations
2013

HHM motif at the CuH-Site of peptidylglycine monooxygenase is a pH-dependent conformational switch

Kline, C. D., Mayfield, M. & Blackburn, N. J., Apr 16 2013, In : Biochemistry. 52, 15, p. 2586-2596 11 p.

Research output: Contribution to journalArticle

14 Scopus citations

Interdomain long-range electron transfer becomes rate-limiting in the Y216A variant of tyramine β-monooxygenase

Osborne, R. L., Zhu, H., Iavarone, A. T., Blackburn, N. J. & Klinman, J. P., Feb 19 2013, In : Biochemistry. 52, 7, p. 1179-1191 13 p.

Research output: Contribution to journalArticle

13 Scopus citations
2012

Alternative ground states enable pathway switching in biological electron transfer

Abriata, L. A., Álvarez-Paggi, D., Ledesma, G. N., Blackburn, N. J., Vila, A. J. & Murgida, D. H., Oct 23 2012, In : Proceedings of the National Academy of Sciences of the United States of America. 109, 43, p. 17348-17353 6 p.

Research output: Contribution to journalArticle

23 Scopus citations

Lumenal loop M672-P707 of the menkes protein (ATP7A) transfers copper to peptidylglycine monooxygenase

Otoikhian, A., Barry, A. N., Mayfield, M., Nilges, M., Huang, Y., Lutsenko, S. & Blackburn, N. J., Jun 27 2012, In : Journal of the American Chemical Society. 134, 25, p. 10458-10468 11 p.

Research output: Contribution to journalArticle

15 Scopus citations

Metal Export by CusCFBA, the Periplasmic Cu(I)/Ag(I) Transport System of Escherichia coli

Mealman, T. D., Blackburn, N. J. & McEvoy, M. M., 2012, Current Topics in Membranes. Academic Press Inc., p. 163-196 34 p. (Current Topics in Membranes; vol. 69).

Research output: Chapter in Book/Report/Conference proceedingChapter

26 Scopus citations

N-Terminal region of CusB is sufficient for metal binding and metal transfer with the metallochaperone CusF

Mealman, T. D., Zhou, M., Affandi, T., Chacón, K. N., Aranguren, M. E., Blackburn, N. J., Wysocki, V. H. & McEvoy, M. M., Aug 28 2012, In : Biochemistry. 51, 34, p. 6767-6775 9 p.

Research output: Contribution to journalArticle

25 Scopus citations
27 Scopus citations
2011

A copper-methionine interaction controls the pH-dependent activation of peptidylglycine monooxygenase

Bauman, A. T., Broers, B. A., Kline, C. D. & Blackburn, N. J., Dec 20 2011, In : Biochemistry. 50, 50, p. 10819-10828 10 p.

Research output: Contribution to journalArticle

21 Scopus citations
22 Scopus citations

The lumenal loop Met 672-Pro 707 of copper-transporting ATPase ATP7A binds metals and facilitates copper release from the intramembrane sites

Barry, A. N., Otoikhian, A., Bhatt, S., Shinde, U., Tsivkovskii, R., Blackburn, N. J. & Lutsenko, S., Jul 29 2011, In : Journal of Biological Chemistry. 286, 30, p. 26585-26594 10 p.

Research output: Contribution to journalArticle

28 Scopus citations
2010

Anatomy of a red copper center: Spectroscopic identification and reactivity of the copper centers of bacillus subtilis Sco and its Cys-to-ala variants

Siluvai, G. S., Mayfield, M., Nilges, M. J., Debeer George, S. & Blackburn, N. J., Apr 14 2010, In : Journal of the American Chemical Society. 132, 14, p. 5215-5226 12 p.

Research output: Contribution to journalArticle

31 Scopus citations

A Tale of Two Metals

Blackburn, N. J., Jan 29 2010, In : Chemistry and Biology. 17, 1, p. 8-9 2 p.

Research output: Contribution to journalShort survey

5 Scopus citations

Interactions between copper-binding sites determine the redox status and conformation of the regulatory N-terminal domain of ATP7B

LeShane, E. S., Shinde, U., Walker, J. M., Barry, A. N., Blackburn, N. J., Ralle, M. & Lutsenko, S., Feb 26 2010, In : Journal of Biological Chemistry. 285, 9, p. 6327-6336 10 p.

Research output: Contribution to journalArticle

28 Scopus citations

The copper centers of tyramine β-monooxygenase and its catalytic-site methionine variants: An X-ray absorption study

Hess, C. R., Klinman, J. P. & Blackburn, N. J., Nov 2010, In : Journal of Biological Inorganic Chemistry. 15, 8, p. 1195-1207 13 p.

Research output: Contribution to journalArticle

19 Scopus citations

Transforming a blue copper into a red copper protein: Engineering cysteine and homocysteine into the axial position of azurin using site-directed mutagenesis and expressed protein ligation

Clark, K. M., Yu, Y., Marshall, N. M., Sieracki, N. A., Nilges, M. J., Blackburn, N. J., Van Der Donk, W. A. & Lu, Y., Jul 28 2010, In : Journal of the American Chemical Society. 132, 29, p. 10093-10101 9 p.

Research output: Contribution to journalArticle

49 Scopus citations
2009

H135A controls the redox activity of the Sco copper center. Kinetic and spectroscopic studies of the His135Ala variant of Bacillus subtilis Sco

Siluvai, G. S., Nakano, M. M., Mayfield, M., Nilges, M. J. & Blackburn, N. J., Dec 29 2009, In : Biochemistry. 48, 51, p. 12133-12144 12 p.

Research output: Contribution to journalArticle

24 Scopus citations

Three-dimensional structure of the human copper transporter hCTR1

De Feo, C. J., Aller, S. G., Siluvai, G. S., Blackburn, N. J. & Unger, V. M., Mar 17 2009, In : Proceedings of the National Academy of Sciences of the United States of America. 106, 11, p. 4237-4242 6 p.

Research output: Contribution to journalArticle

187 Scopus citations

Tryptophan Cu(I)-π interaction fine-tunes the metal binding properties of the bacterial metallochaperone CusF

Loftin, I. R., Blackburn, N. J. & McEvoy, M. M., 2009, In : Journal of Biological Inorganic Chemistry. 14, 6, p. 905-912 8 p.

Research output: Contribution to journalArticle

26 Scopus citations
2008
17 Scopus citations

Direct metal transfer between periplasmic proteins identifies a bacterial copper chaperone

Bagai, I., Rensing, C., Blackburn, N. J. & McEvoy, M. M., Nov 4 2008, In : Biochemistry. 47, 44, p. 11408-11414 7 p.

Research output: Contribution to journalArticle

86 Scopus citations

Selenocysteine positional variants reveal contributions to copper binding from cysteine residues in domains 2 and 3 of human copper chaperone for superoxide dismutase

Barry, A. N., Clark, K. M., Otoikhian, A., Van Der Donk, W. A. & Blackburn, N. J., Dec 9 2008, In : Biochemistry. 47, 49, p. 13074-13083 10 p.

Research output: Contribution to journalArticle

13 Scopus citations

Structural studies of copper(I) complexes of amyloid-β peptide fragments: Formation of two-coordinate bis(histidine) complexes

Himes, R. A., Park, G. Y., Siluvai, G. S., Blackburn, N. J. & Karlin, K. D., Nov 10 2008, In : Angewandte Chemie - International Edition. 47, 47, p. 9084-9087 4 p.

Research output: Contribution to journalArticle

109 Scopus citations
2007

A multinuclear copper(I) cluster forms the dimerization interface in copper-loaded human copper chaperone for superoxide dismutase

Stasser, J. P., Siluvai, G. S., Barry, A. N. & Blackburn, N. J., Oct 23 2007, In : Biochemistry. 46, 42, p. 11845-11856 12 p.

Research output: Contribution to journalArticle

41 Scopus citations

Large scale production of the copper enzyme peptidylglycine monooxygenase using an automated bioreactor

Bauman, A. T., Ralle, M. & Blackburn, N. J., Jan 1 2007, In : Protein Expression and Purification. 51, 1, p. 34-38 5 p.

Research output: Contribution to journalArticle

10 Scopus citations

Substrate-linked conformational change in the periplasmic component of a Cu(I)/Ag(I) efflux system

Bagai, I., Liu, W., Rensing, C., Blackburn, N. J. & McEvoy, M. M., Dec 7 2007, In : Journal of Biological Chemistry. 282, 49, p. 35695-35702 8 p.

Research output: Contribution to journalArticle

71 Scopus citations

Synthesis and x-ray absorption spectroscopy structural studies of Cu(I) complexes of HistidylHistidine peptides: The predominance of linear 2-coordinate geometry

Himes, R. A., Ga, Y. P., Barry, A. N., Blackburn, N. J. & Karlin, K. D., May 2 2007, In : Journal of the American Chemical Society. 129, 17, p. 5352-5353 2 p.

Research output: Contribution to journalArticle

78 Scopus citations

Unusual Cu(I)/Ag(I) coordination of Escherichia coli CusF as revealed by atomic resolution crystallography and X-ray absorption spectroscopy

Loftin, I. R., Franke, S., Blackburn, N. J. & Mcevoy, M. M., Oct 2007, In : Protein Science. 16, 10, p. 2287-2293 7 p.

Research output: Contribution to journalArticle

93 Scopus citations