Francis Valiyaveetil

Assistant Professor

  • 1038 Citations
  • 14 h-Index
19972018

Research Output 1997 2018

  • 1038 Citations
  • 14 h-Index
  • 25 Article
  • 3 Chapter
  • 1 Comment/debate
2018

Patch-clamp recordings of the KcsA K+ channel in unilamellar blisters

Matulef, K. & Valiyaveetil, F. I. 2018 Methods in Molecular Biology. Humana Press Inc., Vol. 1684, p. 181-191 11 p. (Methods in Molecular Biology; vol. 1684)

Research output: ResearchChapter

Blister
Ion Channels
Unilamellar Liposomes
Electrophysiology
Xenopus
2017

A glimpse into the C-type-inactivated state for a potassium channel

Valiyaveetil, F. I. Oct 5 2017 In : Nature Structural and Molecular Biology. 24, 10, p. 787-788 2 p.

Research output: Research - peer-reviewComment/debate

Probing the Effects of Gating on the Ion Occupancy of the K+ Channel Selectivity Filter Using Two-Dimensional Infrared Spectroscopy

Kratochvil, H. T., Maj, M., Matulef, K., Annen, A. W., Ostmeyer, J., Perozo, E., Roux, B., Valiyaveetil, F. I. & Zanni, M. T. Jul 5 2017 In : Journal of the American Chemical Society. 139, 26, p. 8837-8845 9 p.

Research output: Research - peer-reviewArticle

Molecular dynamics
Infrared spectroscopy
Ions
Molecular Dynamics Simulation
Spectrum Analysis
2016
3 Citations

Combining in Vitro Folding with Cell Free Protein Synthesis for Membrane Protein Expression

Focke, P. J., Hein, C., Hoffmann, B., Matulef, K., Bernhard, F., Dötsch, V. & Valiyaveetil, F. I. Aug 2 2016 In : Biochemistry. 55, 30, p. 4212-4219 8 p.

Research output: Research - peer-reviewArticle

Membrane Proteins
Proteins
In Vitro Techniques
Precipitates
Amino Acid Transport Systems
17 Citations

Instantaneous ion configurations in the K+ion channel selectivity filter revealed by 2D IR spectroscopy

Kratochvil, H. T., Carr, J. K., Matulef, K., Annen, A. W., Li, H., Maj, M., Ostmeyer, J., Serrano, A. L., Raghuraman, H., Moran, S. D., Skinner, J. L., Perozo, E., Roux, B., Valiyaveetil, F. I. & Zanni, M. T. Sep 2 2016 In : Science. 353, 6303, p. 1040-1044 5 p.

Research output: Research - peer-reviewArticle

Ion Channels
Spectrum Analysis
Ions
Binding Sites
Potassium Channels
2015
3 Citations

Engineering the glutamate transporter homologue GltPh using protein semisynthesis

Focke, P. J., Annen, A. W. & Valiyaveetil, F. I. Mar 3 2015 In : Biochemistry. 54, 8, p. 1694-1702 9 p.

Research output: Research - peer-reviewArticle

Amino Acid Transport System X-AG
Substrates
Proteins
Peptides
Binding Sites
2 Citations

Incorporation of non-canonical amino acids

Leisle, L., Valiyaveetil, F., Mehl, R. A. & Ahern, C. A. Sep 17 2015 Novel Chemical Tools to Study Ion Channel Biology. Springer New York, p. 119-151 33 p.

Research output: ResearchChapter

Membrane Proteins
Amino Acids
Proteins
Amino Acyl-tRNA Synthetases
Xenopus laevis
5 Citations

Incorporation of non-canonical amino acids

Leisle, L., Valiyaveetil, F., Mehl, R. A. & Ahern, C. A. Oct 1 2015 In : Advances in Experimental Medicine and Biology. 869, p. 119-151 33 p.

Research output: Research - peer-reviewArticle

Membrane Proteins
Amino Acids
Proteins
Amino Acyl-tRNA Synthetases
Xenopus laevis
7 Citations

Individual Ion Binding Sites in the K+ Channel Play Distinct Roles in C-type Inactivation and in Recovery from Inactivation

Matulef, K., Annen, A. W., Nix, J. C. & Valiyaveetil, F. I. Dec 29 2015 (Accepted/In press) In : Structure.

Research output: Research - peer-reviewArticle

Binding Sites
Ions
Amides
Esters
Proteins
8 Citations

Ion-binding properties of a K+ channel selectivity filter in different conformations

Liu, S., Focke, P. J., Matulef, K., Bian, X., Moënne-Loccoz, P., Valiyaveetil, F. I. & Lockless, S. W. Dec 8 2015 In : Proceedings of the National Academy of Sciences of the United States of America. 112, 49, p. 15096-15100 5 p.

Research output: Research - peer-reviewArticle

Ions
Lipid Bilayers
Intracellular Membranes
Calorimetry
Streptomyces
2013
2 Citations

Engineering K+ channels using semisynthesis

Komarov, A. G., Costantino, C. A. & Valiyaveetil, F. I. 2013 Methods in Molecular Biology. Vol. 995, p. 3-17 15 p. (Methods in Molecular Biology; vol. 995)

Research output: ResearchChapter

Potassium Channels
Site-Directed Mutagenesis
Membrane Proteins
Binding Sites
Ions
25 Citations

Semisynthetic K+ channels show that the constricted conformation of the selectivity filter is not the C-type inactivated state

Devaraneni, P. K., Komarov, A. G., Costantino, C. A., Devereaux, J. J., Matulef, K. & Valiyaveetil, F. I. Sep 24 2013 In : Proceedings of the National Academy of Sciences of the United States of America. 110, 39, p. 15698-15703 6 p.

Research output: Research - peer-reviewArticle

Alanine
Proteins
Voltage-Gated Potassium Channels
Glycine
Amino Acids
14 Citations

Using protein backbone mutagenesis to dissect the link between ion occupancy and C-Type inactivation in K+ channels

Matulef, K., Komarov, A. G., Costantino, C. A. & Valiyaveetil, F. I. Oct 29 2013 In : Proceedings of the National Academy of Sciences of the United States of America. 110, 44, p. 17886-17891 6 p.

Research output: Research - peer-reviewArticle

Mutagenesis
Esters
Ions
Proteins
Amides
2011
7 Citations

In vitro folding of K vAP, a voltage-gated K + channel

Devaraneni, P. K., Devereaux, J. J. & Valiyaveetil, F. I. Dec 6 2011 In : Biochemistry. 50, 48, p. 10442-10450 9 p.

Research output: Research - peer-reviewArticle

Voltage-Gated Potassium Channels
Temperature
In Vitro Techniques
Chemical analysis
Assays
2010
6 Citations

Semisynthesis of NaK, a Na+ and K+ conducting ion channel

Linn, K. M., Derebe, M. G., Jiang, Y. & Valiyaveetil, F. I. Jun 1 2010 In : Biochemistry. 49, 21, p. 4450-4456 7 p.

Research output: Research - peer-reviewArticle

Ion Channels
Peptides
Ions
Ligation
Homoserine
9 Citations

Studies of ion channels using expressed protein ligation

Focke, P. J. & Valiyaveetil, F. I. Dec 2010 In : Current Opinion in Chemical Biology. 14, 6, p. 797-802 6 p.

Research output: Research - peer-reviewArticle

Ion Channels
Ligation
Proteins
Peptides
Porins
2009
4 Citations

Chapter 7 Semisynthesis of K+ Channels

Komarov, A. G., Linn, K. M., Devereaux, J. J. & Valiyaveetil, F. I. 2009 In : Methods. 462, p. 135-150 16 p.

Research output: Research - peer-reviewArticle

Ions
Mutagenesis
Binding Sites
Site-Directed Mutagenesis
Membrane Proteins
27 Citations

Modular strategy for the semisynthesis of a K+ channel: Investigating interactions of the pore helix

Komarov, A. G., Linn, K. M., Devereaux, J. J. & Valiyaveetil, F. I. Dec 18 2009 In : ACS Chemical Biology. 4, 12, p. 1029-1038 10 p.

Research output: Research - peer-reviewArticle

Tryptophan
Membrane Proteins
Hydrogen
Peptides
Proteins
2006
95 Citations

Ion selectivity in a semisynthetic K+ channel locked in the conductive conformation

Valiyaveetil, F. I., Leonetti, M., Muir, T. W. & MacKinnon, R. Nov 10 2006 In : Science. 314, 5801, p. 1004-1007 4 p.

Research output: Research - peer-reviewArticle

Potassium
Ions
Potassium Channels
Porins
Crystallography
35 Citations

Structural and functional consequences of an amide-to-ester substitution in the selectivity filter of a potassium channel

Valiyaveetil, F. I., Sekedat, M., MacKinnon, R. & Muir, T. W. Sep 6 2006 In : Journal of the American Chemical Society. 128, 35, p. 11591-11599 9 p.

Research output: Research - peer-reviewArticle

Potassium Channels
Amides
Esters
Substitution reactions
Ions
2004
66 Citations

Glycine as a D-amino acid surrogate in the K+-selectivity filter

Valiyaveetil, F. I., Sekedat, M., MacKinnon, R. & Muir, T. W. Dec 7 2004 In : Proceedings of the National Academy of Sciences of the United States of America. 101, 49, p. 17045-17049 5 p.

Research output: Research - peer-reviewArticle

Glycine
Amino Acids
42 Citations

Semisynthesis of a functional K+ channel

Valiyaveetil, F. I., Sekedat, M., Muir, T. W. & MacKinnon, R. May 3 2004 In : Angewandte Chemie - International Edition. 43, 19, p. 2504-2507 4 p.

Research output: Research - peer-reviewArticle

Ions
Chemical modification
Membrane Lipids
Atoms
2002
249 Citations

Lipids in the structure, folding, and function of the KcsA K+ channel

Valiyaveetil, F. I., Zhou, Y. & MacKinnon, R. Sep 3 2002 In : Biochemistry. 41, 35, p. 10771-10777 7 p.

Research output: Research - peer-reviewArticle

Lipids
Ion Channels
Molecules
Potassium Channels
Immunoglobulin Fab Fragments
19 Citations
Dicyclohexylcarbodiimide
Detergents
Escherichia coli
Membranes
F1F0-ATP synthase
117 Citations

Semisynthesis and folding of the potassium channel KcsA

Valiyaveetil, F. I., MacKinnon, R. & Muir, T. W. Aug 7 2002 In : Journal of the American Chemical Society. 124, 31, p. 9113-9120 8 p.

Research output: Research - peer-reviewArticle

Potassium Channels
Proteins
Potassium
Ligation
Peptides
1998
14 Citations

Subunit interactions coupling H+ transport and ATP synthesis in F1F0 ATP synthase

Fillingame, R. H., Girvin, M. E., Jiang, W., Valiyaveetil, F. & Hermolin, J. 1998 In : Acta Physiologica Scandinavica, Supplement. 163, 643, p. 163-168 6 p.

Research output: Research - peer-reviewArticle

51 Citations

Subunit organization and structure in the F0 sector of Escherichia coli F1F0 ATP synthase

Fillingame, R. H., Jones, P. C., Jiang, W., Valiyaveetil, F. I. & Dmitriev, O. Y. Jun 10 1998 In : Biochimica et Biophysica Acta - Bioenergetics. 1365, 1-2, p. 135-142 8 p.

Research output: Research - peer-reviewArticle

Dimers
Crosslinking
Escherichia coli
Nuclear magnetic resonance
F1F0-ATP synthase
120 Citations

Transmembrane topography of subunit a in the Escherichia coli F1F0 ATP synthase

Valiyaveetil, F. I. & Fillingame, R. H. Jun 26 1998 In : Journal of Biological Chemistry. 273, 26, p. 16241-16247 7 p.

Research output: Research - peer-reviewArticle

Labeling
Topography
Escherichia coli
F1F0-ATP synthase
fluorescein 5-maleimide
1997
91 Citations

On the role of Arg-210 and Glu-219 of subunit α in proton translocation by the Escherichia coli F0F1-ATP synthase

Valiyaveetil, F. I. & Fillingame, R. H. Dec 19 1997 In : Journal of Biological Chemistry. 272, 51, p. 32635-32641 7 p.

Research output: Research - peer-reviewArticle

Escherichia coli
Protons
Adenosine Triphosphate
Membranes
Genes