David Farrens

Associate Professor

  • 4824 Citations
  • 34 h-Index
1989 …2019
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Research Output 1989 2019

  • 4824 Citations
  • 34 h-Index
  • 63 Article
  • 2 Chapter
2019
30 Citations (Scopus)

Structure of a Signaling Cannabinoid Receptor 1-G Protein Complex

Krishna Kumar, K., Shalev-Benami, M., Robertson, M. J., Hu, H., Banister, S. D., Hollingsworth, S. A., Latorraca, N. R., Kato, H. E., Hilger, D., Maeda, S., Weis, W. I., Farrens, D., Dror, R. O., Malhotra, S. V., Kobilka, B. K. & Skiniotis, G., Jan 24 2019, In : Cell. 176, 3, p. 448-458.e12

Research output: Contribution to journalArticle

Cannabinoid Receptors
GTP-Binding Proteins
Chemical activation
Heterotrimeric GTP-Binding Proteins
Cannabinoids

Visualizing endogenous opioid receptors in living neurons using ligand-directed chemistry

Arttamangkul, S., Plazek, A., Platt, E. J., Jin, H., Murray, T. F., Birdsong, W. T., Rice, K. C., Farrens, D. L. & Williams, J. T., Oct 7 2019, In : eLife. 8

Research output: Contribution to journalArticle

Open Access
Opioid Receptors
Neurons
Ligands
Fluorescent Dyes
Labeling
2018
4 Citations (Scopus)

Single Proteoliposome High Content Analysis Reveals Differences in the Homo-Oligomerization of GPCRs

Walsh, S. M., Mathiasen, S., Christensen, S. M., Fay, J. F., King, C., Provasi, D., Borrero, E., Rasmussen, S. G. F., Fung, J. J., Filizola, M., Hristova, K., Kobilka, B., Farrens, D. & Stamou, D., Jan 1 2018, (Accepted/In press) In : Biophysical Journal.

Research output: Contribution to journalArticle

Hominidae
G-Protein-Coupled Receptors
CCR10 Receptors
Opsins
Cannabinoid Receptors
2017
3 Citations (Scopus)

Purification of Functional CB1 and Analysis by Site-Directed Fluorescence Labeling Methods

Fay, J. F. & Farrens, D., 2017, Methods in Enzymology. Academic Press Inc., Vol. 593. p. 343-370 28 p. (Methods in Enzymology; vol. 593).

Research output: Chapter in Book/Report/Conference proceedingChapter

Labeling
Purification
Fluorescence
Cannabinoids
Ligands
2016
100 Citations (Scopus)

Biosensor reveals multiple sources for mitochondrial NAD+

Cambronne, X., Stewart, M. L., Kim, D., Jones-Brunette, A. M., Morgan, R. K., Farrens, D., Cohen, M. & Goodman, R., Jun 17 2016, In : Science. 352, 6292, p. 1474-1477 4 p.

Research output: Contribution to journalArticle

Biosensing Techniques
NAD
Sirtuins
Mitochondria
Enzymes
16 Citations (Scopus)
G-Protein-Coupled Receptors
Rhodopsin
Hydroxylamine
Schiff Bases
Tryptophan
5 Citations (Scopus)

Evidence that the Rhodopsin Kinase (GRK1) N-Terminus and the Transducin Gα C-Terminus Interact with the Same "hydrophobic Patch" on Rhodopsin TM5

Jones Brunette, A. M., Sinha, A., David, L. & Farrens, D., Jun 7 2016, In : Biochemistry. 55, 22, p. 3123-3135 13 p.

Research output: Contribution to journalArticle

G-Protein-Coupled Receptor Kinase 1
Transducin
Rhodopsin
Phosphotransferases
G-Protein-Coupled Receptors
2015
12 Citations (Scopus)

Conformational selection and equilibrium governs the ability of retinals to bind opsin

Schafer, C. T. & Farrens, D., Feb 13 2015, In : Journal of Biological Chemistry. 290, 7, p. 4304-4318 15 p.

Research output: Contribution to journalArticle

Opsins
Retinaldehyde
Conformations
Chemical activation
Arrestin
7 Citations (Scopus)

Retinal attachment instability is diversified among mammalian melanopsins

Tsukamoto, H., Kubo, Y., Farrens, D., Koyanagi, M., Terakita, A. & Furutani, Y., Nov 6 2015, In : Journal of Biological Chemistry. 290, 45, p. 27176-27187 12 p.

Research output: Contribution to journalArticle

Mammals
Invertebrates
Mutagenesis
Retinal Pigments
Schiff Bases
17 Citations (Scopus)
Cannabinoid Receptor CB1
GTP-Binding Proteins
Ligands
Allosteric Site
Arrestin
18 Citations (Scopus)

The Enzymatic Activity of Lipases Correlates with Polarity-Induced Conformational Changes: A Trp-Induced Quenching Fluorescence Study

Skjold-Jørgensen, J., Bhatia, V. K., Vind, J., Svendsen, A., Bjerrum, M. J. & Farrens, D., Jun 18 2015, In : Biochemistry. 54, 27, p. 4186-4196 11 p.

Research output: Contribution to journalArticle

Lipase
Quenching
Esterases
Fluorescence
Enzyme Activation
2014
19 Citations (Scopus)

Distance mapping in proteins using fluorescence spectroscopy: Tyrosine, like tryptophan, quenches bimane fluorescence in a distance-dependent manner

Jones Brunette, A. M. & Farrens, D., Oct 14 2014, In : Biochemistry. 53, 40, p. 6290-6301 12 p.

Research output: Contribution to journalArticle

Fluorescence Spectrometry
Fluorescence spectroscopy
Tryptophan
Tyrosine
Quenching
40 Citations (Scopus)

Fluorescence spectroscopy of rhodopsins: Insights and approaches

Alexiev, U. & Farrens, D., 2014, In : Biochimica et Biophysica Acta - Bioenergetics. 1837, 5, p. 694-709 16 p.

Research output: Contribution to journalArticle

Rhodopsin
Fluorescence Spectrometry
Fluorescence spectroscopy
Fluorescence
Proteins
37 Citations (Scopus)

Nanoscale high-content analysis using compositional heterogeneities of single proteoliposomes

Mathiasen, S., Christensen, S. M., Fung, J. J., Rasmussen, S. G. F., Fay, J. F., Jorgensen, S. K., Veshaguri, S., Farrens, D., Kiskowski, M., Kobilka, B. & Stamou, D., 2014, In : Nature Methods. 11, 9, p. 931-934 4 p.

Research output: Contribution to journalArticle

Assays
Oligomerization
Fluorescence microscopy
Fluorescence Microscopy
Adrenergic Receptors
15 Citations (Scopus)

Rhodopsin TM6 can interact with two separate and distinct sites on arrestin: Evidence for structural plasticity and multiple docking modes in arrestin-rhodopsin binding

Sinha, A., Jones Brunette, A. M., Fay, J. F., Schafer, C. T. & Farrens, D., May 27 2014, In : Biochemistry. 53, 20, p. 3294-3307 14 p.

Research output: Contribution to journalArticle

Arrestin
Rhodopsin
Plasticity
Fingers
Transducin
2013
23 Citations (Scopus)
G-Protein-Coupled Receptors
Ligands
Opsins
Mutation
Labeling
21 Citations (Scopus)

The membrane proximal region of the cannabinoid receptor CB1 N-terminus can allosterically modulate ligand affinity

Fay, J. F. & Farrens, D., Nov 19 2013, In : Biochemistry. 52, 46, p. 8286-8294 9 p.

Research output: Contribution to journalArticle

Cannabinoid Receptor CB1
Disulfides
Ligands
Membranes
Cysteine
2012
30 Citations (Scopus)

A key agonist-induced conformational change in the cannabinoid receptor CB1 is blocked by the allosteric ligand Org 27569

Fay, J. F. & Farrens, D., Sep 28 2012, In : Journal of Biological Chemistry. 287, 40, p. 33873-33882 10 p.

Research output: Contribution to journalArticle

Cannabinoid Receptor CB1
Chemical activation
Ligands
G-Protein-Coupled Receptors
Labeling
1 Citation (Scopus)

Contributions of H G Khorana to understanding transmembrane signal transduction

Farrens, D. & Sakmar, T. P., 2012, In : Resonance. 17, 12, p. 1165-1173 9 p.

Research output: Contribution to journalArticle

large family
co-worker
retirement
biology
2011
17 Citations (Scopus)

Rhodopsin in nanodiscs has native membrane-like photointermediates

Tsukamoto, H., Szundi, I., Lewis, J. W., Farrens, D. & Kliger, D. S., Jun 7 2011, In : Biochemistry. 50, 22, p. 5086-5091 6 p.

Research output: Contribution to journalArticle

Rhodopsin
Membranes
Detergents
Photoexcitation
Light scattering
2010
53 Citations (Scopus)

Distance mapping in proteins using fluorescence spectroscopy: The tryptophan-induced quenching (TrIQ) method

Mansoor, S. E., Dewitt, M. A. & Farrens, D., Nov 16 2010, In : Biochemistry. 49, 45, p. 9722-9731 10 p.

Research output: Contribution to journalArticle

Fluorescence Spectrometry
Fluorescence spectroscopy
Tryptophan
Quenching
Proteins
68 Citations (Scopus)

Monomeric rhodopsin is the minimal functional unit required for arrestin binding

Tsukamoto, H., Sinha, A., DeWitt, M. & Farrens, D., Jun 2010, In : Journal of Molecular Biology. 399, 3, p. 501-511 11 p.

Research output: Contribution to journalArticle

Arrestin
Rhodopsin
G-Protein-Coupled Receptors
Liposomes
Phospholipids
21 Citations (Scopus)
Rhodopsin
GTP-Binding Proteins
Labeling
marking
Chemical activation
2009
40 Citations (Scopus)

The magnitude of the light-induced conformational change in different rhodopsins correlates with their ability to activate G proteins

Tsukamoto, H., Farrens, D., Koyanagi, M. & Terakita, A., Jul 31 2009, In : Journal of Biological Chemistry. 284, 31, p. 20676-20683 8 p.

Research output: Contribution to journalArticle

Rhodopsin
GTP-Binding Proteins
Light
G-Protein-Coupled Receptors
Quenching
2007
41 Citations (Scopus)

Dynamics of arrestin-rhodopsin interactions: Loop movement is involved in arrestin activation and receptor binding

Sommer, M. E., Farrens, D., McDowell, J. H., Weber, L. A. & Smith, W. C., Aug 31 2007, In : Journal of Biological Chemistry. 282, 35, p. 25560-25568 9 p.

Research output: Contribution to journalArticle

Arrestin
Rhodopsin
Chemical activation
Cysteine
Quenching
21 Citations (Scopus)

The Conformations of the Manganese Transport Regulator of Bacillus subtilis in its Metal-free State

DeWitt, M. A., Kliegman, J. I., Helmann, J. D., Brennan, R. G., Farrens, D. & Glasfeld, A., Feb 2 2007, In : Journal of Molecular Biology. 365, 5, p. 1257-1265 9 p.

Research output: Contribution to journalArticle

Manganese
Bacillus subtilis
Metals
DNA
Selenomethionine
2006
30 Citations (Scopus)

Arrestin can act as a regulator of rhodopsin photochemistry

Sommer, M. E. & Farrens, D., Dec 2006, In : Vision Research. 46, 27, p. 4532-4546 15 p.

Research output: Contribution to journalArticle

Arrestin
Photochemistry
Rhodopsin
Night Blindness
Transducin
264 Citations (Scopus)

Coupling ligand structure to specific conformational switches in the β2-adrenoceptor

Yao, X., Parnot, C., Deupi, X., Ratnala, V. R. P., Swaminath, G., Farrens, D. & Kobilka, B., Aug 2006, In : Nature Chemical Biology. 2, 8, p. 417-422 6 p.

Research output: Contribution to journalArticle

Adrenergic Receptors
G-Protein-Coupled Receptors
Ligands
Rhodopsin
Fluorescence Spectrometry
29 Citations (Scopus)

Dynamics of arrestin-rhodopsin interactions: Acidic phospholipids enable binding of arrestin to purified rhodopsin in detergent

Sommer, M. E., Smith, W. C. & Farrens, D., Apr 7 2006, In : Journal of Biological Chemistry. 281, 14, p. 9407-9417 11 p.

Research output: Contribution to journalArticle

Arrestin
Rhodopsin
Detergents
Phospholipids
Micelles
78 Citations (Scopus)

Rhodopsin self-associates in asolectin liposomes

Mansoor, S. E., Palczewski, K. & Farrens, D., Feb 28 2006, In : Proceedings of the National Academy of Sciences of the United States of America. 103, 9, p. 3060-3065 6 p.

Research output: Contribution to journalArticle

Rhodopsin
Liposomes
Energy Transfer
Luminescence
Transducin
2005
44 Citations (Scopus)
rimonabant
Cannabinoid Receptors
Cysteine
Labeling
Chemical activation
62 Citations (Scopus)

Dynamics of arrestin-rhodopsin interactions: Arrestin and retinal release are directly linked events

Sommer, M. E., Smith, W. C. & Farrens, D., Feb 25 2005, In : Journal of Biological Chemistry. 280, 8, p. 6861-6871 11 p.

Research output: Contribution to journalArticle

Arrestin
Rhodopsin
Light
Fluorescence
Retinal Rod Photoreceptor Cells

Structural dynamics of the signal termination process in rhodopsin

Farrens, D., 2005, Light Sensing in Plants. Springer Japan, p. 203-212 10 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

rhodopsin
Rhodopsin
arrestins
Light Signal Transduction
phototransduction
2004
44 Citations (Scopus)

High-throughput protein structural analysis using site-directed fluorescence labeling and the bimane derivative (2-pyridyl)dithiobimane

Mansoor, S. E. & Farrens, D., Jul 27 2004, In : Biochemistry. 43, 29, p. 9426-9438 13 p.

Research output: Contribution to journalArticle

Structural analysis
Labeling
Fluorescence
Throughput
Derivatives
90 Citations (Scopus)

Rhodopsin activation exposes a key hydrophobic binding site for the transducin α-subunit C terminus

Janz, J. M. & Farrens, D., Jul 9 2004, In : Journal of Biological Chemistry. 279, 28, p. 29767-29773 7 p.

Research output: Contribution to journalArticle

Transducin
Rhodopsin
Chemical activation
Binding Sites
Fluorescence
53 Citations (Scopus)

Role of the retinal hydrogen bond network in rhodopsin Schiff base stability and hydrolysis

Janz, J. M. & Farrens, D., Dec 31 2004, In : Journal of Biological Chemistry. 279, 53, p. 55886-55894 9 p.

Research output: Contribution to journalArticle

Rhodopsin
Schiff Bases
Hydrogen
Hydrolysis
Hydrogen bonds
2003
25 Citations (Scopus)
Rhodopsin
Schiff Bases
Opsins
Retinal Diseases
Retinal Pigments
69 Citations (Scopus)

Stability of dark state rhodopsin is mediated by a conserved ion pair in intradiscal loop E-2

Janz, J. M., Fay, J. F. & Farrens, D., May 9 2003, In : Journal of Biological Chemistry. 278, 19, p. 16982-16991 10 p.

Research output: Contribution to journalArticle

Rhodopsin
Ions
Hydrolysis
Retinaldehyde
Opsins
2002
19 Citations (Scopus)

Design, expression, and characterization of a synthetic human cannabinoid receptor and cannabinoid receptor/G-protein fusion protein

Farrens, D., Dunham, T. D., Fay, J. F., Dews, I. C., Caldwell, J. & Nauert, B., Dec 1 2002, In : Journal of Peptide Research. 60, 6, p. 336-347 12 p.

Research output: Contribution to journalArticle

Cannabinoid Receptors
GTP-Binding Proteins
Fusion reactions
Genes
Proteins
73 Citations (Scopus)

Mapping proximity within proteins using fluorescence spectroscopy. A study of T4 lysozyme showing that tryptophan residues quench bimane fluorescence

Mansoor, S. E., Mchaourab, H. S. & Farrens, D., Feb 26 2002, In : Biochemistry. 41, 8, p. 2475-2484 10 p.

Research output: Contribution to journalArticle

Fluorescence Spectrometry
Fluorescence spectroscopy
Muramidase
Tryptophan
Fluorescence
2001
294 Citations (Scopus)

Agonist-induced conformational changes in the G-protein-coupling domain of the β2 adrenergic receptor

Ghanouni, P., Steenhuis, J. J., Farrens, D. & Kobilka, B. K., May 22 2001, In : Proceedings of the National Academy of Sciences of the United States of America. 98, 11, p. 5997-6002 6 p.

Research output: Contribution to journalArticle

Fluorescein
GTP-Binding Proteins
Adrenergic Receptors
G-Protein-Coupled Receptors
Fluorescence
52 Citations (Scopus)

Consensus and Variant cAMP-regulated Enhancers Have Distinct CREB-binding Properties

Craig, J. C., Schumacher, M. A., Mansoor, S. E., Farrens, D., Brennan, R. G. & Goodman, R., Apr 13 2001, In : Journal of Biological Chemistry. 276, 15, p. 11719-11728 10 p.

Research output: Contribution to journalArticle

Cyclic AMP Response Element-Binding Protein
Protein Binding
Dimerization
DNA
Ions
33 Citations (Scopus)

Engineering a functional blue-wavelength-shifted rhodopsin mutant

Janz, J. M. & Farrens, D., Jun 19 2001, In : Biochemistry. 40, 24, p. 7219-7227 9 p.

Research output: Contribution to journalArticle

Rhodopsin
Wavelength
Mutation
Transducin
Opsins
318 Citations (Scopus)

Functionally Different Agonists Induce Distinct Conformations in the G Protein Coupling Domain of the β2 Adrenergic Receptor

Ghanouni, P., Gryczynski, Z., Steenhuis, J. J., Lee, T. W., Farrens, D., Lakowicz, J. R. & Kobilka, B. K., Jul 6 2001, In : Journal of Biological Chemistry. 276, 27, p. 24433-24436 4 p.

Research output: Contribution to journalArticle

GTP-Binding Proteins
Adrenergic Receptors
Conformations
G-Protein-Coupled Receptors
Ligands
1999
159 Citations (Scopus)
Rhodopsin
Fluorescence Spectrometry
Fluorescence spectroscopy
Labeling
Cysteine
50 Citations (Scopus)
Secondary Protein Structure
Muramidase
Fluorescent Dyes
Labeling
Fluorescence
182 Citations (Scopus)

Domains responsible for constitutive and Ca2+-dependent interactions between calmodulin and small conductance Ca2+-activated potassium channels

Keen, J. E., Khawaled, R., Farrens, D., Neelands, T., Rivard, A., Bond, C. T., Janowsky, A., Fakler, B., Adelman, J. & Maylie, J., Oct 15 1999, In : Journal of Neuroscience. 19, 20, p. 8830-8838 9 p.

Research output: Contribution to journalArticle

Potassium Channels
Calmodulin
Small-Conductance Calcium-Activated Potassium Channels
Ion Channel Gating
Hand
81 Citations (Scopus)

Single-cysteine substitution mutants at amino acid positions 306-321 in rhodopsin, the sequence between the cytoplasmic end of helix VII and the palmitoylation sites: Sulfhydryl reactivity and transducin activation reveal a tertiary structure

Cai, K., Klein-Seetharaman, J., Farrens, D., Zhang, C., Altenbach, C., Hubbell, W. L. & Gobind Khorana, H., Jun 22 1999, In : Biochemistry. 38, 25, p. 7925-7930 6 p.

Research output: Contribution to journalArticle

Lipoylation
Transducin
Rhodopsin
Cysteine
Substitution reactions
1996
1067 Citations (Scopus)

Requirement of rigid-body motion of transmembrane helices for light activation of rhodopsin

Farrens, D., Altenbach, C., Yang, K., Hubbell, W. L. & Khorana, H. G., Nov 1 1996, In : Science. 274, 5288, p. 768-770 3 p.

Research output: Contribution to journalArticle

Rhodopsin
Cysteine
Light
Transducin
Spin Labels
274 Citations (Scopus)

Structural features and light-dependent changes in the cytoplasmic interhelical E-F loop region of rhodopsin: A site-directed spin-labeling study

Altenbach, C., Yang, K., Farrens, D., Farahbakhsh, Z. T., Gobind Khorana, H. & Hubbell, W. L., 1996, In : Biochemistry. 35, 38, p. 12470-12478 9 p.

Research output: Contribution to journalArticle

Rhodopsin
Labeling
Electron Spin Resonance Spectroscopy
Light
Paramagnetic resonance